Figure S1. SDS-PAGE analysis of purified TdPMEI proteins produced in Escherichia coli.

Figure S2. Western blot of purified recombinant PMEIs performed by using an Anti-His antibody.

Figure S3. 15% SDS-PAGE of purified TdPMEI recombinant proteins in reducing and not reducing conditions.

Figure S4. Chromosomal assignment of Tdpmei2.1 based on PCR products obtained with primer pair TapmeiFor/TapmeiRev, spanning the complete coding region of the gene.

Figure S5. PCR amplicons and sequence homology of wheat and wild progenitors.

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