Figure S1. Nucleotide sequence of the Nt-Ub1 cDNA and its deduced amino acid sequence.

Figure S2. A: Alignment of amino acid sequence of Nt-Ub1 (Nicotiana tabacum, DQ830978) with Ubi.U4 (N.  tabacum, X77456) and Rubq1 (Oryza sativa, U37687). Identical amino acid residues in this alignment are shaded in black. B: Phylogenetic relationship of nucleic acid sequence between Nt-Ub1 (N.  tabacum, DQ830978), Ubi.U4 (N.  tabacum, X77456), Ta-Ub2 (Triticum aestivum, AY297059) and Rubq1 (O.  sativa, U37687). The tree was generated using DNAman version 4.0 (Lynnon BioSoft Co., USA).

Figure S3. Primary structure of the antisense 35S-Nt-Ub1 gene.

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