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Table S1. AFLP, MSAP and cDNA-AFLP adapters, primers and selective primer combinations used in this study.

Table S1. Molecular characterisation of fragments showing methylation changes in the allohexaploids and/or the hybrids relative to their parents.

Table S1. Molecular characterisation of transcript-derived fragments showing different expression in the allohexaploids and/or the hybrids according to cDNA–AFLP analysis.

Figure S1. Examples of AFLP analysis in the triploid hybrids F1 (ArBcCc), derived allohexaploids S1 (ArArBcBcCcCc), and their progenitors Brassica carinata (BBCC) and Brassica rapa (AA). Arrow indicated genetic alteration in the allohexaploids.

Figure S2. Examples of MSAP analysis in the triploid hybrids F1 (ArBcCc), derived allohexaploids S1 (ArArBcBcCcCc), and their progenitors B. carinata (BBCC) and B. rapa (AA). H: fragments obtained after digestion with EcoRIHpaII; M: fragments obtained after digestion with EcoRIMspI. Arrows indicated DNA methylation alteration.

Figure S3. Examples of cDNA-AFLP analysis in the triploid hybrids F1 (ArBcCc), derived allohexaploids S1 (ArArBcBcCcCc), and their progenitors B. carinata (BBCC) and B. rapa (AA). Arrow indicated gene silencing in the hybrid but reactivated in the allohexaploids.

FilenameFormatSizeDescription
PLB_553_sm_TableS1.doc45KSupporting info item
PLB_553_sm_TableS2.doc135KSupporting info item
PLB_553_sm_TableS3.doc128KSupporting info item
PLB_553_sm_fig-S1.tif733KSupporting info item
PLB_553_sm_fig-S2.tif1808KSupporting info item
PLB_553_sm_fig-S3.tif844KSupporting info item

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