Editor E. Flemetakis
Shortening tobacco life cycle accelerates functional gene identification in genomic research
Article first published online: 26 MAR 2012
© 2012 German Botanical Society and The Royal Botanical Society of the Netherlands
Volume 14, Issue 6, pages 934–943, November 2012
How to Cite
Ning, G., Xiao, X., Lv, H., Li, X., Zuo, Y. and Bao, M. (2012), Shortening tobacco life cycle accelerates functional gene identification in genomic research. Plant Biology, 14: 934–943. doi: 10.1111/j.1438-8677.2012.00571.x
- Issue published online: 29 OCT 2012
- Article first published online: 26 MAR 2012
- Received: 8 November 2011; Accepted: 22 January 2012
Figure S1. Schematic diagram showing thestrategy and primers used to isolate genomic DNA and cDNA sequencesof the PmFT gene using FPNI-PCR.
Figure S2. FT orthologue from P.mume (PmFT gene).
Figure S3. Genomic PCR or RT-PCR analysis toconfirm putative transgenic plants and expression of PmFT orPtFT in selected transgenic tobacco plants with various early flowering phenotypes.
Figure S4. Cross and longitudinal stem sectionsof wild-type (Wt) and transgenic early-flowering tobaccoplants.
Figure S5. Comparison of petal epidermal cells in the different transgenic tobacco lines through SEM observation.
Table S1. Primers used for the generation of a648-bp genomic conservative fragment of the P. mume FTorthologue and for isolation of the full-length PmFT cDNA.
Table S2. Gene-specific primers used inFPNI-PCR for generation of the PmFT genomic fragmentcontaining the PmFT exon and intron sequences.
Table S3. Random primers used for generation ofthe PmFT genomic fragment in FPNI-PCR experiments.
Table S4. Genomic PCR or RT-PCR primers used for analysis of putative transgenic plants.
|plb571_sm_FigS1.jpg||98K||Supporting info item|
|plb571_sm_FigS2.jpg||260K||Supporting info item|
|plb571_sm_FigS3.jpg||119K||Supporting info item|
|plb571_sm_FigS4.jpg||517K||Supporting info item|
|plb571_sm_FigS5.jpg||274K||Supporting info item|
|plb571_sm_supportinglegends.doc||37K||Supporting info item|
|plb571_sm_TableS1-S4.doc||53K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.