Figure S1. Schematic diagram showing thestrategy and primers used to isolate genomic DNA and cDNA sequencesof the PmFT gene using FPNI-PCR.

Figure S2. FT orthologue from P.mume (PmFT gene).

Figure S3. Genomic PCR or RT-PCR analysis toconfirm putative transgenic plants and expression of PmFT orPtFT in selected transgenic tobacco plants with various early flowering phenotypes.

Figure S4. Cross and longitudinal stem sectionsof wild-type (Wt) and transgenic early-flowering tobaccoplants.

Figure S5. Comparison of petal epidermal cells in the different transgenic tobacco lines through SEM observation.

Table S1. Primers used for the generation of a648-bp genomic conservative fragment of the P. mume FTorthologue and for isolation of the full-length PmFT cDNA.

Table S2. Gene-specific primers used inFPNI-PCR for generation of the PmFT genomic fragmentcontaining the PmFT exon and intron sequences.

Table S3. Random primers used for generation ofthe PmFT genomic fragment in FPNI-PCR experiments.

Table S4. Genomic PCR or RT-PCR primers used for analysis of putative transgenic plants.

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