Editor R. Mendel
Isolation and characterisation of an antifolate insensitive (afi1) mutant of Arabidopsis thaliana
Article first published online: 5 JUN 2012
© 2012 German Botanical Society and The Royal Botanical Society of the Netherlands
Volume 15, Issue 1, pages 37–44, January 2013
How to Cite
Navarrete, O., Van Daele, J., Stove, C., Lambert, W., Storozhenko, S. and Van Der Straeten, D. (2013), Isolation and characterisation of an antifolate insensitive (afi1) mutant of Arabidopsis thaliana. Plant Biology, 15: 37–44. doi: 10.1111/j.1438-8677.2012.00602.x
- Issue published online: 28 NOV 2012
- Article first published online: 5 JUN 2012
- Received: 22 December 2011; Accepted: 9 March 2012
Figure S1. Folic acid (FA) levels in Arabidopsis seedlings treated with TMP and MTX.
Figure S2. 5-Methyl-THF, 5-formyl-THF and THF levels in Arabidopsis seedlings treated with TMP and MTX.
Figure S3. THF, 5,10-methylene-THF (5,10-CH-THF), 5-formyl-THF (5-CHO), 10-formyl-THF (10-CHO) and 5-methyl-THF (5-CH3-THF) levels in Arabidopsis seedlings grown on different concentrations of TMP.
Figure S4. Expression analysis of folate-related genes in the afi1 mutant upon MTX treatment.
Table S1. List of primers used in this study for mapping and semi-quantitative PCR.
Table S2. Results from the mapping analysis in all the chromosomes from the cross afi1 × Ler in the F2 population.
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