These authors contributed equally to this work.
Functional analysis of a WRKY transcription factor involved in transcriptional activation of the DBAT gene in Taxus chinensis
Article first published online: 12 JUN 2012
© 2012 German Botanical Society and The Royal Botanical Society of the Netherlands
Volume 15, Issue 1, pages 19–26, January 2013
How to Cite
Li, S., Zhang, P., Zhang, M., Fu, C. and Yu, L. (2013), Functional analysis of a WRKY transcription factor involved in transcriptional activation of the DBAT gene in Taxus chinensis. Plant Biology, 15: 19–26. doi: 10.1111/j.1438-8677.2012.00611.x
Editor J. Whelan
- Issue published online: 28 NOV 2012
- Article first published online: 12 JUN 2012
- Received: 25 November 2011; Accepted: 16 March 2012
- Taxus chinensis cells;
Although the regulation of taxol biosynthesis at the transcriptional level remains unclear, 10-deacetylbaccatin III-10 β-O-acetyl transferase (DBAT) is a critical enzyme in the biosynthesis of taxol. The 1740 bp fragment 5′-flanking sequence of the dbat gene was cloned from Taxus chinensis cells. Important regulatory elements needed for activity of the dbat promoter were located by deletion analyses in T. chinensis cells. A novel WRKY transcription factor, TcWRKY1, was isolated with the yeast one-hybrid system from a T. chinensis cell cDNA library using the important regulatory elements as bait. The gene expression of TcWRKY1 in T. chinensis suspension cells was specifically induced by methyl jasmonate (MeJA). Biochemical analysis indicated that TcWRKY1 protein specifically interacts with the two W-box (TGAC) cis-elements among the important regulatory elements. Overexpression of TcWRKY1 enhanced dbat expression in T. chinensis suspension cells, and RNA interference (RNAi) reduced the level of transcripts of dbat. These results suggest that TcWRKY1 participates in regulation of taxol biosynthesis in T. chinensis cells, and that dbat is a target gene of this transcription factor. This research also provides a potential candidate gene for engineering increased taxol accumulation in Taxus cell cultures.