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Fig. S1. CLSM sections through the nuclei (A–C) and projections of 20 sections (D–F) of A.  nidus leaf protoderm after α-tubulin (A and D) and TPX2 (B and E) immunostaining, and their combination (C and F). TPX2 signal coincides with perinuclear microtubules in prophase cells (asterisks in A and B) and with the metaphase spindle (arrowheads in A and B). Scale bar = 10 μm.

Fig. S2. CLSM sections of prophase cells after α-tubulin immunostaining. In these specimens cold methanol treatment (see Material and Methods) was omitted. The peculiar contour of the nuclei (asterisks) resembles those of cells at the same stage in this study. Scale bar = 10 μm.

Fig. S3. Fluorescence microscopy images of A.  nidus dividing cells after α-tubulin (A and D) and TPX2 (B and E) immunostaining, and DNA counterstaining with Hoechst 33258 (C and F). A–C: TPX2 (B) is co-localised with the microtubules (A) of the metaphase spindle (arrows in A and B) and phragmoplast (arrowheads in A and B). The arrow in (C) points to the metaphasic chromosomes and the arrowheads to the daughter chromosome groups. See also the TPX2 signal (B) around the prophase nucleus (asterisks in A–C), coincident with perinuclear microtubules (A). D–F: TPX2 co-localisation (E) with the microtubules (D) of the anaphase spindle (arrows in D and E) in a cell similar to that depicted in Fig. 2G–I. The arrowheads in (F) point to the segregating chromosome groups. See also the TPX2 signal on the new cell wall (arrowhead in E), coincident with the microtubule aggregation (arrowhead in D). Scale bar = 10 μm.

Fig. S4. Projection of 20 CLSM sections of the cell depicted in Fig. 5D–F. As also shown in single CLSM sections, TPX2 signal (B) does not follow the perinuclear microtubules (A, see also overlap in C). Scale bar = 10 μm.

Fig. S1. CLSM sections through the nuclei (A?C) and projections of 20 sections (D?F) of A.  nidus leaf protoderm after ?-tubulin (A and D) and TPX2 (B and E) immunostaining, and their combination (C and F). TPX2 signal coincides with perinuclear microtubules in prophase cells (asterisks in A and B) and with the metaphase spindle (arrowheads in A and B). Scale bar = 10 ?m.

Fig. S2. CLSM sections of prophase cells after ?-tubulin immunostaining. In these specimens cold methanol treatment (see Material and Methods) was omitted. The peculiar contour of the nuclei (asterisks) resembles those of cells at the same stage in this study. Scale bar = 10 ?m.

Fig. S3. Fluorescence microscopy images of A.  nidus dividing cells after ?-tubulin (A and D) and TPX2 (B and E) immunostaining, and DNA counterstaining with Hoechst 33258 (C and F). A?C: TPX2 (B) is co-localised with the microtubules (A) of the metaphase spindle (arrows in A and B) and phragmoplast (arrowheads in A and B). The arrow in (C) points to the metaphasic chromosomes and the arrowheads to the daughter chromosome groups. See also the TPX2 signal (B) around the prophase nucleus (asterisks in A?C), coincident with perinuclear microtubules (A). D?F: TPX2 co-localisation (E) with the microtubules (D) of the anaphase spindle (arrows in D and E) in a cell similar to that depicted in Fig. 2G?I. The arrowheads in (F) point to the segregating chromosome groups. See also the TPX2 signal on the new cell wall (arrowhead in E), coincident with the microtubule aggregation (arrowhead in D). Scale bar = 10 ?m.

Fig. S4. Projection of 20 CLSM sections of the cell depicted in Fig. 5D?F. As also shown in single CLSM sections, TPX2 signal (B) does not follow the perinuclear microtubules (A, see also overlap in C). Scale bar = 10 ?m.

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plb615_sm_FigS4.jpg2234KSupporting info item
plb615_sm_Figure-legends.doc36KSupporting info item

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