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Fig. S1. Location of populations (samplingareas) of Acanthorrhynchium papillatum in Malaysia: SB– Sungei Bantang Recreational Forest; GB – GunungBelumut Forest Reserve; KT – Kota Tinggi Waterfalls Resort. Kuala Lumpur (KL) is shown for reference.

Fig. S2. Habitat pictures of populations ofAcanthorrhynchium papillatum in Malaysia: (A) Sungei Bantang Recreational Forest; (B) Gunung Belumut Forest Reserve; (C) Kota Tinggi Waterfalls Resort.

Fig. S3. Location of populations ofAcanthorrhynchium papillatum in Singapore: BT – BukitTimah Nature Reserve; MR – MacRitchie Reservoir.

Fig. S4. Habitat pictures of populations ofAcanthorrhynchium papillatum in Singapore: (A) Bukit Timah Nature Reserve; (B) MacRitchie Reservoir.

Fig. S5. Neighbour-joining tree constructed from allele sharing distances of samples from Sungei Bantang Recreation Forest, Malaysia.

Fig. S6. Neighbour-joining tree constructed from allele sharing distances of samples from Gunung Belumut Forest Reserve, Malaysia. Samples with asterisks had genotypes that matched other samples, as shown in Table 3.

Fig. S7. Neighbour-joining tree constructed from allele sharing distances of samples from MacRitchie Reservoir, Singapore.

Table S1. Approximate pair-wise distancesbetween populations (sampling areas) of Acanthorrhynchiumpapillatum.

Table S2. Custom-run module parameters that were optimised to allow for fragment analysis runs using Data Collection software version 1 of the PRISM 3100 Genetic Analyser (Applied Biosystems, USA).

Table S3. Microsatellite marker alleles of samples for among-clump diversity studies. Header rows indicate names of markers.

Table S4. Allelic frequencies of all microsatellite markers used for each population. Alleles are amplicon lengths in bp.

Table S5. Size ranges of microsatellite alleles for each of the eight loci and for each population.

Table S6. Number of alleles per locus for each population before (B) and after rarefaction (R).

Table S7. Private alleles per population after rarefaction. Base sampling size was set at 31 individuals.

Table S8. The distribution of samples with null alleles for each marker and for every population.

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