plb685-sup-0001-FigS1.tifimage/tif261KFigure S1. Indole-3-acetic acid (IAA) quantification and auxin-responsive gene expression. (A) IAA quantification. Free IAA levels were measured in 5-week-old Col-0 and lines overexpressing IBR3 plants (OE-1, OE-2 and OE-3) using GCMS. Data represent average values ± SE of three independent biological replicates. (B–E) Expression levels of auxin-responsive genes upon Pst DC3000 infection in transgenics overexpressing IBR3. Five-week-old Col-0 and lines overexpressing IBR3 (OE-1, OE-2 and OE-3) were dip-inoculated with 5 × 10cfu ml−1 Pst DC3000. AUX1 (B), PIN1 (C), GH3.5 (D) and IAA29 (E) expression levels were determined by qRT-PCR at 0 and 18 hpi. Expression levels were normalised to both UBQ10 and EF-1. Relative gene expression levels were compared to Col-0 at 0 hpi with a defined expression value of 1. Data represent means ± SE of three independent biological replicates, each consisting of three technical replicates (n = 9). hpi, hours post-inoculation.

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