Molecular characterisation of a calmodulin gene, VcCaM1, that is differentially expressed under aluminium stress in highbush blueberry

Authors

  • C. Inostroza-Blancheteau,

    1. Núcleo de Investigación en Producción Alimentaria, Escuela de Agronomía, Facultad de Recursos Naturales, Universidad Católica de Temuco, Temuco, Chile
    2. Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco, Chile
    3. Laboratorio de Bioquímica, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia, Universidad Católica de Chile, Santiago, Chile
    Search for more papers by this author
  • F. Aquea,

    1. Laboratorio de Bioquímica, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia, Universidad Católica de Chile, Santiago, Chile
    Search for more papers by this author
  • R. Loyola,

    1. Laboratorio de Bioquímica, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia, Universidad Católica de Chile, Santiago, Chile
    Search for more papers by this author
  • J. Slovin,

    1. Genetic Improvement of Fruits and Vegetables Laboratory, U.S. Department of Agriculture-Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Beltsville, MD, USA
    Search for more papers by this author
  • S. Josway,

    1. Genetic Improvement of Fruits and Vegetables Laboratory, U.S. Department of Agriculture-Agricultural Research Service, Henry A. Wallace Beltsville Agricultural Research Center, Beltsville, MD, USA
    Search for more papers by this author
  • Z. Rengel,

    1. Soil Science and Plant Nutrition, School of Earth and Environment, The University of Western Australia, Crawley, WA, Australia
    Search for more papers by this author
  • M. Reyes-Díaz,

    1. Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco, Chile
    2. Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Temuco, Chile
    Search for more papers by this author
  • M. Alberdi,

    1. Center of Plant, Soil Interaction and Natural Resources Biotechnology, Scientific and Technological Bioresource Nucleus (BIOREN), Universidad de La Frontera, Temuco, Chile
    2. Departamento de Ciencias Químicas y Recursos Naturales, Universidad de La Frontera, Temuco, Chile
    Search for more papers by this author
  • P. Arce-Johnson

    Corresponding author
    1. Laboratorio de Bioquímica, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia, Universidad Católica de Chile, Santiago, Chile
    • Correspondence

      P. Arce-Johnson, Laboratorio de Bioquímica, Departamento de Genética Molecular y Microbiología, Facultad de Ciencias Biológicas, Pontificia Universidad Católica de Chile, PO Box 114-D Santiago, Chile.

      E-mail: parce@bio.puc.cl

    Search for more papers by this author

Abstract

Calmodulin (CaM), a small acidic protein, is one of the best characterised Ca2+ sensors in eukaryotes. This Ca2+-regulated protein plays a critical role in decoding and transducing environmental stress signals by activating specific targets. Many environmental stresses elicit changes in intracellular Ca2+ activity that could initiate adaptive responses under adverse conditions. We report the first molecular cloning and characterisation of a calmodulin gene, VcCaM1 (Vaccinium corymbosum Calmodulin 1), in the woody shrub, highbush blueberry. VcCaM1 was first identified as VCAL19, a gene induced by aluminium stress in V. corymbosum L. A full-length cDNA of VcCaM1 containing a 766-bp open reading frame (ORF) encoding 149 amino acids was cloned from root RNA. The sequence encodes four Ca2+-binding motifs (EF-hands) and shows high similarity (99%) with the isoform CaM 201 of Daucus carota. Expression analyses showed that following Al treatment, VcCaM1 message level decreased in roots of Brigitta, an Al-resistant cultivar, and after 48 h, was lower than in Bluegold, an Al-sensitive cultivar. VcCAM1 message also decreased in leaves of both cultivars within 2 h of treatment. Message levels in leaves then increased by 24 h to control levels in Brigitta, but not in Bluegold, but then decreased again by 48 h. In conclusion, VcCaM1 does not appear to be directly involved in Al resistance, but may be involved in improved plant performance under Al toxicity conditions through regulation of Ca2+ homeostasis and antioxidant systems in leaves.

Ancillary