Separation of sub-populations of sperm with higher fertility potential from normal and pathological semen by peanut agglutinin

Trennung von Spermatozoensubpopulationen mit einem höheren Fertilitäts-Potential von normalem und pathologischem Sperma mittels des Erdnußagglutinins

Authors

  • A. Ravid,

    1. Rogoff Medical Research Institute and The Male Fertility Laboratory, Beilinson Medical Center, Petah-Tikva, The Department of Life Sciences, Bar-Ilan University, Ramat-Gan and The Connective Tissue Research Unit, Hasharon Medical Center, Petah-Tikva, Israel
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  • M. Sagiv,

    1. Rogoff Medical Research Institute and The Male Fertility Laboratory, Beilinson Medical Center, Petah-Tikva, The Department of Life Sciences, Bar-Ilan University, Ramat-Gan and The Connective Tissue Research Unit, Hasharon Medical Center, Petah-Tikva, Israel
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  • B. Bartoov,

    1. Rogoff Medical Research Institute and The Male Fertility Laboratory, Beilinson Medical Center, Petah-Tikva, The Department of Life Sciences, Bar-Ilan University, Ramat-Gan and The Connective Tissue Research Unit, Hasharon Medical Center, Petah-Tikva, Israel
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  • F. Eltes,

    1. Rogoff Medical Research Institute and The Male Fertility Laboratory, Beilinson Medical Center, Petah-Tikva, The Department of Life Sciences, Bar-Ilan University, Ramat-Gan and The Connective Tissue Research Unit, Hasharon Medical Center, Petah-Tikva, Israel
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  • A. Novogrodsky,

    1. Rogoff Medical Research Institute and The Male Fertility Laboratory, Beilinson Medical Center, Petah-Tikva, The Department of Life Sciences, Bar-Ilan University, Ramat-Gan and The Connective Tissue Research Unit, Hasharon Medical Center, Petah-Tikva, Israel
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  • D. Allalouf,

    1. Rogoff Medical Research Institute and The Male Fertility Laboratory, Beilinson Medical Center, Petah-Tikva, The Department of Life Sciences, Bar-Ilan University, Ramat-Gan and The Connective Tissue Research Unit, Hasharon Medical Center, Petah-Tikva, Israel
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  • H. Levinsky,

    1. Rogoff Medical Research Institute and The Male Fertility Laboratory, Beilinson Medical Center, Petah-Tikva, The Department of Life Sciences, Bar-Ilan University, Ramat-Gan and The Connective Tissue Research Unit, Hasharon Medical Center, Petah-Tikva, Israel
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  • R. Singer

    Corresponding author
    1. Rogoff Medical Research Institute and The Male Fertility Laboratory, Beilinson Medical Center, Petah-Tikva, The Department of Life Sciences, Bar-Ilan University, Ramat-Gan and The Connective Tissue Research Unit, Hasharon Medical Center, Petah-Tikva, Israel
      Male Fertility Laboratory, Beilinson Medical Center, Petah-Tikva 49 100 Israel.
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Male Fertility Laboratory, Beilinson Medical Center, Petah-Tikva 49 100 Israel.

Abstract

Summary Incubation of human sperm from semen with counts below 20 × 106/ml with peanut agglutinin (PNA) resulted in agglutination of about 70% of sperm. An inverse correlation was found between non-agglutinating (PNA) sperm and sperm density (r = 0.48, p<0.01) and a direct correlation with acrosome-damaged sperm (r =0.83, p< 0.001). Binding of 125I-PNA to sperm revealed high, and possibly also low affinity binding sites on sperm from both normozoospermic and oligozoospermic origins. Quantitative transmission electron microscopy analysis revealed that agglutinated, PNA+, sperm had lower frequency of acrosomeless spermatozoa than PNA--sperm (44% versus 77%) and higher fertility score (+0.18 ± 0.1 and — 1.5 ± 0.87 respectively, p<0.01). Removal of PNA- sperm from oligozoospermic semen may increase the fertility score of the remaining sperm.

Zusammenfassung Die Inkubation von menschlichen Spermatozoen aus dem Sperma einer Spermatozoendichte unter 20 × 106/ml mit dem Erdnuß-Agglutinin (PNA) führte zur Agglutination von circa 70% der Spermatozoen. Es wurde eine umgekehrte Korrelation zwischen den nicht agglutinierten Spermatozoen und der Spermatozoendichte festgestellt (r = 0, 48; p<0, 01) und eine direkte Korrelation mit Spermatozoen und einem Akrosomschaden beobachtet (r = 0.83; p < 0.001). Die Bindung von 125I-PNA an Spermatozoen offenbarte hohe, aber möglicherweise ebenso niedrige Affinitätsbindungs-Stellen an den Spermatozoen sowohl von Normozoospermie als auch von Oligozoospermie. Die Transmissions-Elektronenmikroskopie wies nach, daß agglutinierte, PNA+-Spermatozoen eine geringere Frequenz von akrosomlosen Spermatozoen hatten als PNA--Spermatozoen (44% gegen 77%) und einen höheren Fertilitäts-Score (+ 0,18 ± 0,1 und 1,5 ± 0,87, p<0,01). Die Entfernung von PNA--Spermatozoen aus dem Sperma einer Oligozoospermie kann den Fertilitäts-Score der zurückbleibenden Spermatozoen heraufsetzen.

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