A previous study (von Brockhusen-Holzer & Curio 1990) had led to the major conclusion that prey-naive Anolis lineatopus hatchlings differ in terms of a “hard-wired” perceptual mechanism underlying the recognition of cricket prey. We reconsider here one result leading to that conclusion since it rested on negative evidence in one treatment group as compared to another. A newly applied discriminant analysis utilizing the complete prey rejection/acceptance data of individuals in the two treatment groups, i.e. cricket-rejectors and prefed cricket-acceptors, again permits the repudiation of a motivation-based alternative hypothesis explaining the rejector-acceptor dichotomy. Thereby we reconfirm the original hypothesis of a “hard-wired”, preprogrammed polyethism underlying prey recognition.