Preliminary results on the effects of grape (Vitis vinifera) seed condensed tannins on in vitro intestinal digestibility of the lupin (Lupinus angustifolius) seed protein fraction in small ruminants

Authors

  • A. M. Bruno-Soares,

    1.  Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Centro de Engenharia dos Biossistemas (CEER), Tapada da Ajuda, Lisboa, Portugal
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  • A. L. Soares-Pereira,

    1.  Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Centro de Engenharia dos Biossistemas (CEER), Tapada da Ajuda, Lisboa, Portugal
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  • T. J. S. Matos,

    1.  Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Centro de Engenharia dos Biossistemas (CEER), Tapada da Ajuda, Lisboa, Portugal
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  • J. M. Ricardo-da-Silva

    1.  Instituto Superior de Agronomia, Universidade Técnica de Lisboa, Laboratório Ferreira Lapa (Sector de Enologia), Tapada da Ajuda, Lisboa, Portugal
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A. M. Bruno-Soares, Instituto Superior de Agronomia (CEER), Technical University of Lisbon, Tapada da Ajuda, 1349-017 Lisboa, Portugal. Tel: +351 213653407; Fax: +351 213653195; E-mail: abs@isa.utl.pt

Summary

Condensed tannins (CT) from grape seeds (Vitis vinifera L.) were added to complex the protein fraction of Lupinus angustifolius seeds. Three CT/protein ratios were used: 96 mg/g (T1), 180 mg/g (T2) and 0 mg/g (T0). The CP losses in the rumen were assessed by the nylon-bag technique and CP intestinal digestibility (CPID) was estimated using an in vitro assay applying a three-step procedure: samples were subject to rumen degradation (in situ, 16 h) and the remaining residues were subject to the digestive enzymes of the abomasum and pancreas in vitro. A positive effect (p < 0.05) of the level of CT on the immediately soluble faction a and the insoluble degradable fraction b was observed between T0 and T2. In the presence of CT the rumen degradation rate was reduced (p < 0.05) from 0.0763/h (T0) to 0.0443 /h (T2). The application of CT showed a reduction (around 10 % for T1) of effective rumen CP degradability. The CPID did not seem to be affected (p > 0.05) by the presence of CT. These findings suggest that the use of grape seed CT might have the potential to improve the efficiency of utilisation of the protein fraction from lupin seeds.

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