Metallothionein levels in sperm of various fish species

Authors

  • I. Fabrik,

    1. Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno
    2. Department of Chemistry, Faculty of Science, Masaryk University, Brno
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  • Z. Svobodova,

    1. Research Institute of Fish Culture and Hydrobiology, University of South Bohemia Ceske Budejovice, Vodnany
    2. Institute of Toxicology, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno
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  • V. Adam,

    1. Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno
    2. Department of Animal Nutrition and Forage Production, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno
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  • S. Krizkova,

    1. Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno
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  • L. Trnkova,

    1. Department of Chemistry, Faculty of Science, Masaryk University, Brno
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  • M. Beklova,

    1. Department of Veterinary Ecology and Environmental Protection, Faculty of Veterinary Hygiene and Ecology, University of Veterinary and Pharmaceutical Sciences, Brno, Czech Republic
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  • M. Rodina,

    1. Research Institute of Fish Culture and Hydrobiology, University of South Bohemia Ceske Budejovice, Vodnany
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  • R. Kizek

    1. Department of Chemistry and Biochemistry, Faculty of Agronomy, Mendel University of Agriculture and Forestry, Brno
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Author’s address: Dr Rene Kizek, Department of Chemistry and Biochemistry, Mendel University of Agriculture and Forestry, Zemedelska 1, 613 00 Brno, Czech Republic.
E-mail: kizek@sci.muni.cz

Summary

In the present paper we determined low molecular heavy metal binding protein called metallothionein (MT) in fish spermatozoa sampled from four fish species (Acipenser baerii, Acipenser ruthenus, Perca fluviatilis and Sander lucioperca). The spermatozoa samples were analyzed with differential pulse voltammetry DPV (Brdicka reaction). The average content of MT in the fish sperm was 0.64 ± 0.55 μm. The highest content of MT was determined in spermatozoa of S. lucioperca (0.96 ± 0.86 μm), followed by P. fluviatilis (0.88 ± 0.11 μm), A. ruthenus (0.57 ± 0.60 μm) and A. baerii (0.43 ± 0.25 μm). The differences in the content of MT was probably related to the differences in age and perhaps to the health status based on the environmental conditions in which the tested specimens developed while also other still unknown factors may have been involved.

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