Physico-biochemical parameters and protein profiles of sperm from beluga Huso huso

Authors

  • P. Li,

    1. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech Republic
    2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, China
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  • M. Rodina,

    1. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech Republic
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  • M. Hulak,

    1. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech Republic
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  • D. Gela,

    1. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech Republic
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  • Z. H. Li,

    1. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech Republic
    2. Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, China
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  • O. Linhart

    1. University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, Zatisi, Vodnany, Czech Republic
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Author’s address: P. Li, University of South Bohemia in Ceske Budejovice, Faculty of Fisheries and Protection of Waters, South Bohemian Research Center of Aquaculture and Biodiversity of Hydrocenoses, 728/II Zatisi, 389 25 Vodnany, Czech Republic; Key Laboratory of Freshwater Biodiversity Conservation and Utilization, Ministry of Agriculture, Yangtze River Fisheries Research Institute, Chinese Academy of Fishery Science, Jingzhou, Hubei, China.
E-mail: pingli06@yahoo.com

Summary

Basic physico-biochemical parameters and protein profiles of sperm from beluga (Huso huso), have been evaluated. The results show a stable spermatozoan velocity (ranging from 136.23 ± 5.63 to 105.78 ± 5.27 μms−1) and motility during 2 min post activation, as well as a long duration of the overall spermatozoa motility period (up to 5 min). Mean values were determined for seminal plasma protein concentration (0.29 ± 0.16 mg ml−1), spermatozoa concentration (0.28 ± 0.27 × 109 spz ml−1), seminal plasma osmolality (51.33 ± 4.91 mOsmol kg−1), the pH (8.49 ± 0.01) and Na+ (18.97 ± 3.65 mm), K+ (2.83 ± 1.36 mm), Ca2+ (0.19 ± 0.06 mm), Mg2+ (0.49 ± 0.23 mm) and Cl (6.33 ± 0.58 mm) concentrations. Moreover, in seminal plasma, five protein bands with molecular weights (MW) of 71, 49, 46, 34, 29 kDa were identified. In spermatozoa, about 100 spots with molecular weights varying from 26.5 to 107 kDa and iso-electric points ranging from 5 to 9.5 were found. The observed physiological and biochemical properties, together with protein patterns, should be considered for the development of methods for controlled reproduction and sperm cryopreservation for the highly endangered beluga sturgeon.

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