We dedicate this work to the memory of Piero Roggero, who left us suddenly and prematurely last June.
Immunological Discrimination of Phytophthora cinnamomi from other Phytophthorae Pathogenic on Chestnut
Article first published online: 26 APR 2004
Journal of Phytopathology
Volume 152, Issue 4, pages 193–199, April 2004
How to Cite
Ferraris, L., Cardinale, F., Valentino, D., Roggero, P. and Tamietti, G. (2004), Immunological Discrimination of Phytophthora cinnamomi from other Phytophthorae Pathogenic on Chestnut. Journal of Phytopathology, 152: 193–199. doi: 10.1111/j.1439-0434.2004.00823.x
- Issue published online: 26 APR 2004
- Article first published online: 26 APR 2004
- Received September 17, 2003; accepted January 7, 2004
- Western blot;
A polyclonal antiserum (A379) against water soluble proteins from Phytophthora cinnamomi mycelium was produced in rabbit. In ELISA, the 1 : 10 000 diluted antiserum revealed only Phytophthora isolates, not allowing a clear-cut discrimination among congenerous species, in spite of a generally higher reactivity on P. cinnamomi proteins. The antiserum gave positive reactions in Western blot analyses against mycelial proteins from nine species of Phytophthora and Pythium sp. (grown on rich media), but not with Rhizoctonia solani, binucleate Rhizoctonia, Verticillium dahliae, Fusarium oxysporum and Cryphonectria parasitica. All Phytophthora species showed common epitopes on proteins of molecular masses 77, 66, 51 and 48 kDa. However, a species-specific protein of 55 kDa was immunodecorated only in P. cinnamomi samples, thus allowing univocal identification of this species. When tested against total proteins from the same fungi grown on water, the antibody revealed diagnostic bands of 55 and 51 kDa in P. cinnamomi only. The antiserum is therefore suitable for the specific identification of P. cinnamomi emerging in distilled water from infected tissues of chestnut, blueberry and azalea.