Figure S1. Phylogeny of Trachemys and allied emydids (RAxML tree, partitioned analysis by gene and codon) based on the sequence information of five nuclear loci (C-mos, ODC, R35, Rag1, Rag2; 3396 bp).

Table S1. Samples used in the present study.

Table S2. Primers used for amplification and sequencing of ND4L, ND4 and cytb.

Table S3. PCR protocols for mitochondrial and nuclear genes.

Table S4. Step-matrix model applied in the parsimony reconstruction of ancestral states with mesquite.

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