Multilocus enzyme electrophoresis analysis and exoenzymatic activity of Candida albicans strains isolated from women with vaginal candidiasis

Authors

  • Patrícia O. Santos,

    1. Department of Morphology, Applied Microbiology Laboratory, Federal University of Sergipe – FUS, Av. Marechal Rondon, s/n, CEP 49100-000, São Cristóvão – SE, Brazil
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  • Juliana O. Melo,

    1. Department of Morphology, Applied Microbiology Laboratory, Federal University of Sergipe – FUS, Av. Marechal Rondon, s/n, CEP 49100-000, São Cristóvão – SE, Brazil
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  • Camila Monique P. B. S. Ponzzes,

    1. Department of Morphology, Applied Microbiology Laboratory, Federal University of Sergipe – FUS, Av. Marechal Rondon, s/n, CEP 49100-000, São Cristóvão – SE, Brazil
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  • José Antônio B. Alves,

    1. Department of Morphology, Applied Microbiology Laboratory, Federal University of Sergipe – FUS, Av. Marechal Rondon, s/n, CEP 49100-000, São Cristóvão – SE, Brazil
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  • Dângelly L.F.M. de Melo,

    1. Department of Morphology, Applied Microbiology Laboratory, Federal University of Sergipe – FUS, Av. Marechal Rondon, s/n, CEP 49100-000, São Cristóvão – SE, Brazil
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  • Nathalia de S. Botelho,

    1. Department of Genetics, Microbiology Laboratory, Estadual University of Londrina – EUL, CEP 86051-990 – Londrina-PR, Brazil
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  • Sueli F. Yamada-Ogatta,

    1. Department of Genetics, Microbiology Laboratory, Estadual University of Londrina – EUL, CEP 86051-990 – Londrina-PR, Brazil
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  • Renata S. Mann,

    1. Department of Agricultural Engineering – FUS, Av. Marechal Rondon, s/n, CEP 49100-000, São Cristóvão-SE, Brazil
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  • Rita de Cássia Trindade

    1. Department of Morphology, Applied Microbiology Laboratory, Federal University of Sergipe – FUS, Av. Marechal Rondon, s/n, CEP 49100-000, São Cristóvão – SE, Brazil
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Patrícia O. Santos, Department of Morphology, Applied Microbiology Laboratory, Federal University of Sergipe – FUS, Av. Marechal Rondon, s/n, CEP 49100-000, São Cristóvão – SE, Brazil.
Tel.: +55 79 2105 6628; Fax: +55 79 2105 6499.
E-mail: oliveira.micro@gmail.com

Summary

Twenty-eight Candida albicans strains obtained from women with vaginal candidiasis were tested for phospholipase and proteinase production and clustered by multilocus enzyme electrophoresis (MLEE). The proteolytic and phospholipidic activity were considered moderate (0.56 ± 0.12 mm and 0.53 ± 0.09 mm, respectively) for all isolates. The isoenzymes malate dehydrogenase (MDH) and sorbitol dehydrogenase (SDH) showed strong intra-specific discriminatory power. The numerical and genetic interpretation of the bands produced by the isoenzymes tested presented similar discriminatory power. The genetic diversity of the isolates was measured by allelic and genic frequency, perceptual index of polymorphic loci (= 87.5%), average number of alleles per locus, average number of alleles per polymorphic locus, average heterozygosity observed and average heterozygosity expected. We verified that three isoenzymatic loci (Adh, Gdh and Sdh-2) were not in Hardy–Weinberg equilibrium. A dendrogram constructed based on the genetic distance matrix of Nei showed seven clusters; 57.15% (16) of the isolates were considered highly related or indistinguishable, and 42.85% were considered moderately related or unrelated. We did not find a relationship between the clusters and the exoenzymes production.

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