Reactivity of the 1,3-β-D-glucan assay during bacteraemia: limited evidence from a prospective study

Authors

  • Zdenek Racil,

    1. Department of Internal Medicine, Hematology and Oncology, Masaryk University and University Hospital Brno, Brno, Czech Republic
    2. CEITEC – Central European Institute of Technology, Masaryk University Brno, Brno, Czech Republic
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  • Iva Kocmanova,

    1. Department of Microbiology, University Hospital Brno, Brno, Czech Republic
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  • Martina Toskova,

    1. Department of Internal Medicine, Hematology and Oncology, Masaryk University and University Hospital Brno, Brno, Czech Republic
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  • Jana Winterova,

    1. Department of Internal Medicine, Hematology and Oncology, Masaryk University and University Hospital Brno, Brno, Czech Republic
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  • Martina Lengerova,

    1. Department of Internal Medicine, Hematology and Oncology, Masaryk University and University Hospital Brno, Brno, Czech Republic
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  • Shira Timilsina,

    1. Department of Internal Medicine, Hematology and Oncology, Masaryk University and University Hospital Brno, Brno, Czech Republic
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  • Jiri Mayer

    1. Department of Internal Medicine, Hematology and Oncology, Masaryk University and University Hospital Brno, Brno, Czech Republic
    2. CEITEC – Central European Institute of Technology, Masaryk University Brno, Brno, Czech Republic
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  • Zdenek Racil and Iva Kocmanova contributed equally to this manuscript.

Zdenek Racil, MD, PhD, Department of Internal Medicine, Hematology and Oncology, University Hospital Brno; Jihlavska 20; 625 00 Brno; Czech Republic.
Tel.: +420 532233642. Fax: +420 532233603.
E-mail: zracil@fnbrno.cz

Summary

There are discrepancies in the retrospective studies published in literature of whether or not bacteraemia could lead to false positivity of 1,3-β-D (BG) glucan assay. We performed, for the first time, a prospective study evaluating the role of bacterial bloodstream infection to the reactivity of BG assay. Twenty-six episodes of bacteraemia that occurred in high-risk haematological patients were included in our study. Consecutive BG levels >80 pg ml−1 were required for test positivity. Only 2 of 26 patients were BG positive – both with IFDs. Thus, we prospectively did not prove bacteraemia as the source of cross reactivity of BG assay in haematological patients.

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