Production of Brassica napus × Raphanobrassica Hybrids by Embryo Rescue: An Attempt to Introduce Shattering Resistance into B. napus

  1. Abha Agnihotri*,
  2. K. R. Shivanna*,
  3. S. N. Raina*,
  4. Malathi Lakshmikumaran*,
  5. Shyam Prakash*,
  6. V. Jagannathan*

Article first published online: 28 APR 2006

DOI: 10.1111/j.1439-0523.1990.tb01288.x

Issue

Plant Breeding

Plant Breeding

Volume 105, Issue 4, pages 292–299, December 1990

Additional Information

How to Cite

Agnihotri, A., Shivanna, K. R., Raina, S. N., Lakshmikumaran, M., Prakash, S. and Jagannathan, V. (1990), Production of Brassica napus × Raphanobrassica Hybrids by Embryo Rescue: An Attempt to Introduce Shattering Resistance into B. napus. Plant Breeding, 105: 292–299. doi: 10.1111/j.1439-0523.1990.tb01288.x

Author Information

  1. Biotechnology Division, Tata Energy Research Institute, New Delhi (India)

* *Two whom correspondence should be addressed.
Abha Agnihotri, Malathi Lakshmikumaran, V. Jagannathan, Tata Energy Research Institute, 90 Jor Bagh, New Delhi 110003; K. R. Shivanna, S. N. Raina, Department of Botany, University of Delhi, Delhi 110007; Shyam Prakash, Nuclear Research Laboratory, Indian Agricultural Research Institute, New Delhi 110012 (India).

Publication History

  1. Issue published online: 28 APR 2006
  2. Article first published online: 28 APR 2006
  3. Received June 22, 1990 / Accepted June 30, 1990

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Keywords:

  • Brassica napus;
  • Raphanobrassica;
  • wide hybridization;
  • embryo rescue;
  • DNA analysis;
  • shattering;
  • resistance;
  • backcrossing

Abstract

Intergeneric hybridization is one of the effective methods to broaden the genetic base of cultivated species. Raphanus sativus, a member of the subtribe Raphaninae, has very hard pods at threshing time. In an attempt to transfer shattering resistance to Brassica napus, Raphanobrassica was used as the male parent in crosses with Brassica napus. Plantlets were obtained by embryo rescue and were further multiplied in vitro by micropropagation of nodal segments. Morphology, cytology and DNA analysis confirmed the hybrid nature of these plants. They were backcrosscd with Brassica napus and the progeny was raised. Plants of BC1 and BC2 generations showed wide variation in morphology, chromosome number and pollen fertility. Some of the plants showed up to 95 % pollen fertility and resistance to shattering, indicating the potential for developing B. napus with resistance to shattering.

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