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Keywords:

  • Brassica fruticulosa;
  • amphiploidy;
  • aphid resistance;
  • genomic affinity;
  • interspecific hybridization;
  • ovary culture

Abstract

An interspecific hybrid between a wild species, Brassica fruticulosa (2n = 16, FF) and a crop Brassica species, B. rapa (2n = 20, AA) was synthesized using sequential ovary-ovule culture on MS medium supplemented with casein hydrolysate. Morphological, molecular and cytogenetic analysis confirmed the true hybrid nature of the offspring. The F1 plants (2n = 18) were intermediate in morphology, highly pollen-sterile as well as self-sterile. A maximum of three bivalents per PMC was recorded, but 14 I + 2 II was the most common meiotic configuration. Normal pollen fertility and regular bivalent (18 II) formation was observed in the amphiploid sectors of the hybrid plants. The F1 hybrid harboured significantly lower aphid populations than the crop Brassica parent.