Development of a high resolution melting (HRM) marker linked to genic male sterility in Capsicum annuum L.
Version of Record online: 22 MAR 2012
© 2012 Blackwell Verlag GmbH
Volume 131, Issue 3, pages 444–448, June 2012
How to Cite
Lee, H.-R., An, H. J., Yang, D. C., Choi, S. H., Kim, H.-J., Rhee, H. G. and Harn, C. H. (2012), Development of a high resolution melting (HRM) marker linked to genic male sterility in Capsicum annuum L. Plant Breeding, 131: 444–448. doi: 10.1111/j.1439-0523.2012.01956.x
- Issue online: 7 JUN 2012
- Version of Record online: 22 MAR 2012
- Received August 2, 2011/Accepted December 31, 2011Communicated by M. Havey
- Capsicum annuum
With 4 figures and 1 table
The characteristics of male sterility (MS) are used in breeding programmes to achieve economical seed production. Male sterility is divided into genic male sterility (GMS) and cytoplasmic male sterility (CMS), which are used to breed commercial pepper varieties. The CMS system, however, is not feasible in some pepper varieties, including bell peppers, because of the absence of a restorer source. GMS is thus important for seed production in bell peppers. In this study, a GMS-linked marker from bell peppers was developed using the bulked segregant analysis (BSA) and amplified fragment length polymorphism (AFLP) method using F2 and sibling individuals. We used 1024 AFLP primer sets and found a polymorphism from EcoRI ACG/MseI GTT among the siblings. An internal sequence-based primer was designed from the 395 bp sequence for high-resolution melting (HRM) analysis, and the marker score of 87 of 92 F2 individuals corresponded to their phenotypes. The marker was mapped on chromosome 5 on the AC99 map.