Early Detection of Pregnancy and Embryonic Loss in Dairy Cattle by ELISA Tests
Article first published online: 12 JUL 2007
Reproduction in Domestic Animals
How to Cite
Gábor, G., Tóth, F., Ózsvári, L., Abonyi-Tóth, Z. and Sasser, R. (2007), Early Detection of Pregnancy and Embryonic Loss in Dairy Cattle by ELISA Tests. Reproduction in Domestic Animals. doi: 10.1111/j.1439-0531.2007.00834.x
- Article first published online: 12 JUL 2007
- Submitted: 29.08.2006
Over a 25-month period 8118 blood samples were assayed for the presence of the serum pregnancy specific-protein B (PSPB) and progesteron (P4) concentrations on three Hungarian large-scale dairy farms. Pregnancy (n = 4085) was checked by BioPRYN assay at 30–36 days post-insemination (PI). Samples from all cows that tested not pregnant and from cows with an optical density (OD) reading in the BioPRYN test that was between 0% and 30% above the cutoff OD value were tested for serum P4 concentration. According to serum P4 concentration, cows were assigned to three categories: high (>4 ng/ml), medium (2–4 ng/ml) and low (<2 ng/ml) serum progesterone. The authors predicted a presumed (low) or possible (medium) late embryonic loss (LEL) or maintenance of the pregnancy (high). A total of 710 LELs were detected (17.4%) and 31.8% of them were predicted because of a low OD value at 30–36 days after insemination. Lower PSPB serum level significantly refers for LEL (p < 0.0001). The prediction rate for the true embryonic loss was 31.8% when OD cutoff from 0% to + 30% of cutoff was examined while it was 62.5% when the threshold was OD cutoff of 0% to 10% of cutoff. The authors conclude that BioPRYN was useful for prediction of a part of LEL in dairy cows and serum P4 concentration in these cows related to the rate of LEL.