DNA Status on Thawed Semen from Fighting Bull: A Comparison Between the SCD and the SCSA Tests

Authors

  • F Martínez-Pastor,

    1. National Wildlife Research Institute (IREC) (UCLM-CSIC-JCCM) and Institute of Regional Development (IDR), Section of Cynegetics and Cattle Resources, University of Castilla-La Mancha, Albacete, Spain
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  • M Del Rocío Fernández-Santos,

    1. National Wildlife Research Institute (IREC) (UCLM-CSIC-JCCM) and Institute of Regional Development (IDR), Section of Cynegetics and Cattle Resources, University of Castilla-La Mancha, Albacete, Spain
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  • ÁE Domínguez-Rebolledo,

    1. National Wildlife Research Institute (IREC) (UCLM-CSIC-JCCM) and Institute of Regional Development (IDR), Section of Cynegetics and Cattle Resources, University of Castilla-La Mancha, Albacete, Spain
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  • MC Esteso,

    1. National Wildlife Research Institute (IREC) (UCLM-CSIC-JCCM) and Institute of Regional Development (IDR), Section of Cynegetics and Cattle Resources, University of Castilla-La Mancha, Albacete, Spain
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  • JJ Garde,

    1. National Wildlife Research Institute (IREC) (UCLM-CSIC-JCCM) and Institute of Regional Development (IDR), Section of Cynegetics and Cattle Resources, University of Castilla-La Mancha, Albacete, Spain
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  • Biology of Reproduction Group

    1. National Wildlife Research Institute (IREC) (UCLM-CSIC-JCCM) and Institute of Regional Development (IDR), Section of Cynegetics and Cattle Resources, University of Castilla-La Mancha, Albacete, Spain
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Errata

This article is corrected by:

  1. Errata: Corrigendum Volume 45, Issue 4, 753, Article first published online: 11 July 2010

Author’s address (for correspondence): F Martínez-Pastor, Grupo de Biología de la Reproducción, IREC, ETSIA, Campus Universitario s/n, 02071 Albacete, Spain. E-mail: felipe.martinez@uclm.es

Contents

The assessment of sperm chromatin status is compulsory in a complete spermiogram. Here we applied the sperm chromatin structure assay (SCSA) and the sperm chromatin dispersion (SCD) test to assess the chromatin status of three fighting bulls. Cryopreserved semen (two straws/bull) were analysed by duplicate after thawing and after 6 h at 37°C with and without oxidative stress (1 mm FE2+). Results (SCD: percentage of spermatozoa with halo; SCSA: SD-DFI, %DFI and HDS) were analysed for differences between bulls and treatments, sensitivity and specificity (receiver operating characteristic curves) and repeatability (repeatability coefficients as 2SD of duplicate differences).%DFI for the three bulls was below 2% at 0 h, indicating no risk for fertility according to previous reports. It increased slightly for two of the bulls after FE2+ treatment (%DFI < 5%) and more pronouncedly for the other bull (C, %DFI∼10%), which merits further investigation. SCD rendered higher percentage of halos for bull C, but could not discriminate between samples with and without oxidizing treatment (AUC: 0.52). SCSA (%DFI) showed a high discriminating ability between treatments (AUC: 0.96). The repeatability coefficient was also higher for SCD (5.9) than for %DFI (1.8), indicating lower repeatability for SCD. Overall, %DFI might be the most useful parameter for assessing sperm chromatin on fighting bull. SCD might yield different information than SCSA, hence further research is warranted.

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