Conflict of interest: MA Hayes declares no conflict of interest; the remaining authors have not declared any conflict of interests.
Proteins Associated With the Early Intrauterine Equine Conceptus
Version of Record online: 9 JUL 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Verlag
Reproduction in Domestic Animals
Special Issue: 16th International Congress on Animal Reproduction
Volume 43, Issue Supplement s2, pages 232–237, July 2008
How to Cite
Hayes, M., Quinn, B., Keirstead, N., Katavolos, P., Waelchli, R. and Betteridge, K. (2008), Proteins Associated With the Early Intrauterine Equine Conceptus. Reproduction in Domestic Animals, 43: 232–237. doi: 10.1111/j.1439-0531.2008.01167.x
- Issue online: 9 JUL 2008
- Version of Record online: 9 JUL 2008
A critical period of early gestation in the mare involves the immobilization (fixation) of the encapsulated conceptus at around days 16–17. We compared the major proteins in the normal equine embryonic capsule and endometrial secretions around the period of fixation with those from pregnancies in the process of termination induced by administration of an analogue of prostaglandin F2α (PGF2α). Uterocalin and β2-microglobulin (β2M) associated with the embryonic capsule were proteolytically converted to smaller forms during the fixation period. These conversions were similar in conceptuses from control and treated mares. A 17 kDa cationic protein identified as a secretory phospholipase A2 (sPLA2) type IIA was detected bound to normal capsules but increased substantially in response to PGF2α. Two forms of uteroglobin were distinguished by partial amino acid sequences of ∼6 kDa bands in flush fluids from normal pregnant uteri. After administration of PGF2α one immunoreactive form of uteroglobin was preferentially increased. These studies demonstrate that failure of pregnancy in this model is associated with an increase in secretory phospholipase in the capsule and a change in the forms of uteroglobin in the uterine secretions.