Comparisons of Different Protocols for Vitrifying Mouse Ovarian Tissue

Authors


Author’s address (for correspondence): Xiao-Ping Wan, Department of Obstetrics and Gynecology, First People’s Hospital Affiliated to Shanghai Jiao Tong University Haining Road 100, Shanghai 200080, China. E-mail: zhang_1974217@163.com

Contents

The aim of this study was to detect the effects of different combinations of cryoprotectants with different equilibrium time on the mouse ovarian tissue during vitrification. Ovarian tissue of mice was vitrified-thawed. Mice (n = 80) were randomly assigned to treatment groups according to different vitrification solutions [I: 20% (v/v) ethylene glycol (EG) + 20% (v/v) Dimethylsulfoxde (DMSO), II: 20% (v/v) EG + 20% (v/v) PROH, III: 20% (v/v) PROH + 20% (v/v) DMSO] and different lengths of equilibrium time (a: 15 min, b: 30 min, c: 45 min). The serum levels of estradiol, the follicular density and the percentage of cells expressing Proliferating-cell nuclear antigen (PCNA) of grafts in Group IIb were the highest in all these treatment groups. In addition, the serum levels of estradiol, the follicular density and the percentage of cells expressing PCNA of grafts in Group Ib were significantly higher than those in Group Ia and Group Ic, while the serum levels of estradiol, the follicular density and the percentage of cells expressing PCNA of grafts in Group IIIb were significantly higher than those in Group IIIa and Group IIIc. In conclusion, vitrification solution [20% (v/v) EG + 20% (v/v) PROH] with equilibrium time of 30 min is optimal selection for vitrifying mouse ovarian tissue.

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