Present address: Department of Animal Nutrition and Feed Management, Poznan University of Life Sciences, Poznan, Poland.
Early Cleaved Bovine Embryos Show Reduced Incidence of Chromosomal Aberrations and Higher Developmental Potential on Day 4.5 Post-Insemination
Article first published online: 4 FEB 2012
© 2012 Blackwell Verlag GmbH
Reproduction in Domestic Animals
Volume 47, Issue 6, pages 899–906, December 2012
How to Cite
Pers-Kamczyc, E., Pawlak, P., Rubes, J. and Lechniak, D. (2012), Early Cleaved Bovine Embryos Show Reduced Incidence of Chromosomal Aberrations and Higher Developmental Potential on Day 4.5 Post-Insemination. Reproduction in Domestic Animals, 47: 899–906. doi: 10.1111/j.1439-0531.2012.01987.x
- Issue published online: 7 NOV 2012
- Article first published online: 4 FEB 2012
- Submitted: 15 Sep 2011; Accepted: 2 Jan 2012
The present study aimed to investigate whether the timing of the first zygotic cleavage (FZC) influences the speed of embryo development expressed by the total cell count and the rate of chromosomally aberrant embryos. Bovine embryos were produced in vitro and divided into two categories according to the timing of FZC: early cleavers (at 30 hpi; EC) and non-early cleavers (at 48 hpi; NEC). On day 4.5 pi, embryos were grouped into three classes depending on the number of blastomeres: delayed (<8 BL), normal (8–16 BL) and advanced (>16 BL). We applied fluorescence in situ hybridization (FISH) with probes for bovine chromosomes 6 and X. The only form of chromosomal imbalance observed was mixoploidy [(2n/3n; 2n/4n); 19.9%, 54/271]. Early cleavers were less often chromosomally unbalanced (13.9%, 20/144) than their NEC counterparts (26.7%, 34/127). Among embryos developing at a normal speed, the NEC embryos were more often abnormal (NEC 20/80; EC 10/79; p < 0.05). The advanced embryos were not observed among the NEC category, whereas such embryos from EC category displayed no chromosomal aberrations. The majority of embryos arrested at the 8 BL stage were of NEC category and were carriers of chromosomally abnormal blastomeres. With regard to embryonic sex, we demonstrated that although males dominate among bovine embryos developing in vitro, the incidence of mixoploidy was equal for both sexes. It can be suggested that a good-quality bovine embryo is usually an early cleaver that develops at higher speed and contains less aberrant cells. The present study also confirmed the usefulness of the FZC as a marker of embryo quality by demonstrating a significantly lower incidence of aberrations in early embryos.