Hypoosmotic Swelling Test in Alpaca (Vicugna pacos) Epididymal Spermatozoa

Authors

  • J Vásquez,

    1. Animal Reproductive Physiology Laboratory, Antonio Raimoindi Biological Sciences Research Institute, Biology Faculty, Universidad Nacional Mayor de San Marcos (UNMSM), Lima, Peru
    Search for more papers by this author
  • EA Florentini,

    1. Animal Reproductive Physiology Laboratory, Antonio Raimoindi Biological Sciences Research Institute, Biology Faculty, Universidad Nacional Mayor de San Marcos (UNMSM), Lima, Peru
    Search for more papers by this author
  • M Valdivia

    1. Animal Reproductive Physiology Laboratory, Antonio Raimoindi Biological Sciences Research Institute, Biology Faculty, Universidad Nacional Mayor de San Marcos (UNMSM), Lima, Peru
    Search for more papers by this author

Author’s address (for correspondence): J. Vásquez, Animal Reproductive Physiology Laboratory, Antonio Raimoindi Biological Sciences Research Institute, Biology Faculty, Universidad Nacional Mayor de San Marcos (UNMSM), Av. Venezuela cda. 34, PO Box 11-0058, Lima 11, Peru. E-mail: jvcbioreprod@gmail.com

Contents

The present study intended to develop the hypoosmotic swelling (HOS) test in alpaca for its use in epididymal spermatozoa, to evaluate the membrane functional integrity and determine an appropriate hypoosmotic solution and whether the incubation time of 15 or 60 min is sufficient for the execution of the test. Hypoosmotic solutions (HS) with the following concentrations were used: 50, 100, 150, 200 and 275 mOsm/kg of sodium citrate tribasic dihydrate and d-fructose. Ten microlitres of epididymal sperm sample was mixed in 150 μL of the respective HS and incubated for 15 or 60 min at 38°C. From the proportion of reacted (swollen) spermatozoa, the 150 mOsm/kg HS was the most sensitive (p < 0.05). The exposure times (15 and 60 min) did not have significant differences (p > 0.05) in the proportion of both strong- and total-coiled sperm tails. In conclusion, 150 mOsm/kg HS and 15 min exposure time are optimal to evaluate the plasma membrane functional integrity through the HOS test in alpaca epididymal spermatozoa.

Ancillary