Prof. HHD Meyer passed away before publication of this manuscript.
Dexamethasone-Induced Eosinopenia is Associated with Lower Progesterone Production in Cattle
Article first published online: 23 MAY 2012
© 2012 Blackwell Verlag GmbH
Reproduction in Domestic Animals
Volume 48, Issue 1, pages 137–148, February 2013
How to Cite
Kliem, H., Rodler, D., Ulbrich, S., Sinowatz, F., Berisha, B., Meyer, H. and Schams, D. (2013), Dexamethasone-Induced Eosinopenia is Associated with Lower Progesterone Production in Cattle. Reproduction in Domestic Animals, 48: 137–148. doi: 10.1111/j.1439-0531.2012.02116.x
- Issue published online: 15 JAN 2013
- Article first published online: 23 MAY 2012
- Submitted: 24 Nov 2011; Accepted: 26 Apr 2012
Eosinophilic cells accumulate in the capillaries of the bovine Graafian follicle shortly before ovulation and in the early developing corpus luteum (CL). Suppressing the migration of these eosinophilic cells by dexamethasone allowed us to evaluate their possible function in the CL development. Brown Swiss cows (n = 10) were randomly subdivided into two groups (n = 5). Every group was used once as control group and once as experimental group with two oestrous cycles between each treatment. Eighteen hours (h) after oestrus synchronization, dexamethasone or saline was given. Ovulation was induced 24 h later with gonadotropin-releasing hormone. Another injection of dexamethasone or saline was given 12 h later. Eosinophilic cells in the blood were counted daily until day 7 after the first dexamethasone injection. The collection of ovaries took place at days 1, 2 and 5. Gene expression, protein concentration and location of angiogenic factors, chemokines, insulin-like growth factor 1 (IGF1) and eosinophilic cells were studied. No eosinophilic cells were found in the CL of the treatment group. Blood progesterone decreased significantly in the dexamethasone group from day 8 to 17. The protein concentration of FGF2 increased significantly in CL tissue at day 2 and VEGFA decreased. Local IGF1 gene expression in the CL was not regulated. We assume from our data that the migration of eosinophilic cells into the early CL is not an essential, but an important stimulus for angiogenesis during early CL development in cattle.