Get access

Postpartum Variations of Plasma IGF and IGFBPs, Oocyte Production and Quality in Dairy Cows: Relationships With Parity and Subsequent Fertility

Authors

  • B Grimard,

    1. UMR 1198 Biologie du Développement et Reproduction, Ecole Nationale Vétérinaire d’Alfort, F-94 704 Maisons-Alfort Cedex, France
    2. UMR 1198 Biologie du Développement et Reproduction, INRA, Domaine de Vilvert, F-78 350 Jouy-en-Josas, France
    Search for more papers by this author
  • B Marquant-Leguienne,

    1. Union Nationale des Coopératives d’Insémination Animale, Recherche et Développement, F-94 700 Maisons-Alfort, France
    Search for more papers by this author
  • D Remy,

    1. UMR 1198 Biologie du Développement et Reproduction, Ecole Nationale Vétérinaire d’Alfort, F-94 704 Maisons-Alfort Cedex, France
    2. UMR 1198 Biologie du Développement et Reproduction, INRA, Domaine de Vilvert, F-78 350 Jouy-en-Josas, France
    Search for more papers by this author
  • C Richard,

    1. INRA, Unité Commune d’Expérimentation Animale, F-91 630 Bressonvilliers, Leudeville, France
    Search for more papers by this author
  • F Nuttinck,

    1. UMR 1198 Biologie du Développement et Reproduction, Ecole Nationale Vétérinaire d’Alfort, F-94 704 Maisons-Alfort Cedex, France
    2. UMR 1198 Biologie du Développement et Reproduction, INRA, Domaine de Vilvert, F-78 350 Jouy-en-Josas, France
    Search for more papers by this author
  • P Humblot,

    1. Union Nationale des Coopératives d’Insémination Animale, Recherche et Développement, F-94 700 Maisons-Alfort, France
    Search for more papers by this author
    • Present address: Faculty of Veterinary Medicine and Animal Science, Swedish University of Agricultural Sciences, SLU, Po Box 7054, SE 750 07 Uppsala, Sweden.

  • AA Ponter

    1. UMR 1198 Biologie du Développement et Reproduction, Ecole Nationale Vétérinaire d’Alfort, F-94 704 Maisons-Alfort Cedex, France
    2. UMR 1198 Biologie du Développement et Reproduction, INRA, Domaine de Vilvert, F-78 350 Jouy-en-Josas, France
    Search for more papers by this author

Author’s address (for correspondence): Bénédicte Grimard, UMR 1198 Biologie du Développement et Reproduction, Ecole Nationale Vétérinaire d’Alfort, 7 avenue du Général de Gaulle, F-94704 Maisons-Alfort Cedex, France. E-mail: bgrimard@vet-alfort.fr

Contents

The aim of this study was to determine whether postpartum variations of plasma IGF-1 and IGFBP concentrations, oocyte production and quality were related to parity and subsequent conception rate in Holstein dairy cows. Holstein dairy cows [10 primiparous (PP) and 22 multiparous (MP)] were allotted in six batches and sampled once weekly between calving and oestrous synchronization treatment started at 71.2 ± 2.0 days postpartum. During the 3 weeks before treatment, ovum pick-up (OPU) was performed twice weekly. Oocytes were scored on a 4-point scale, and oocytes from OPU1, 3 and 5 were fertilized in vitro. Seventeen cows became pregnant after first and second AI and were considered as fertile (F), while the others were considered to be subfertile (SF). Logistic regression was carried out to investigate the relationships between repeated measurements and fertility including parity and batch effects in the models. Likelihood of fertility significantly increased when plasma urea and IGFBP-3 concentrations decreased and was higher in PP compared with MP cows. There was a trend for fertility to increase when plasma IGF-1 concentrations increased (p = 0.07). In vitro cleavage and development rates were similar between SF and F cows (46.4% and 28.3% in SF vs 55.0% and 22.1% in F). Parity had an effect on plasma IGF-1 concentrations (PP: 61.65 ± 2.67 vs MP: 41.63 ± 5.81 ng/ml, p < 0.001), mean number of follicles aspirated per session (PP: 5.7 ± 1.3 vs MP: 9.5 ± 0.8, p < 0.05) and fertility (PP: 8/10 = 80% vs MP: 9/22 = 41%, p < 0.05) but not on the number of oocytes recovered per session nor their quality. In conclusion, postpartum plasma urea and IGFBP-3 concentrations, but not oocyte production and quality before breeding, were related to subsequent conception rate in our experimental design. Parity had a significant effect on energy status, follicular growth and fertility and needs to be considered when investigating relationships between nutrition and reproduction.

Get access to the full text of this article

Ancillary