Present address: Veterinary Clinical Science, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.
Infiltrating Foxp3+ Regulatory T cells and Histopathological Features in Canine Classical and Spermatocytic Seminomas
Article first published online: 7 JUL 2012
© 2012 Blackwell Verlag GmbH
Reproduction in Domestic Animals
Volume 48, Issue 2, pages 218–222, April 2013
How to Cite
Kim, J., Chon, S., Im, K., Kim, N., Cho, K. and Sur, J. (2013), Infiltrating Foxp3+ Regulatory T cells and Histopathological Features in Canine Classical and Spermatocytic Seminomas. Reproduction in Domestic Animals, 48: 218–222. doi: 10.1111/j.1439-0531.2012.02135.x
- Issue published online: 12 MAR 2013
- Article first published online: 7 JUL 2012
- Submitted: 27 Feb 2012; Accepted: 17 May 2012
In humans, regulatory T (T reg) cells are known to play a critical role in both the regulation of immune homoeostasis and the progression of cancer. However, there is little information about the identification, characterization and the function of T reg cells in canine tumours. We identified T reg cells in 28 canine seminoma samples using a Forkhead box P3 (Foxp3) antibody and investigated the relationship between T reg cell infiltration and histopathological features of classical and spermatocytic seminomas (SE and SS, respectively). The Foxp3 protein showed nuclear immunostaining in infiltrating lymphocytes, and Foxp3+ cells were diffused or focally distributed in seminoma tissues. Foxp3+ cells were frequently present in the SS histotype, in seminomas that showed no evidence of tumour cell invasion into the vessels and in seminomas showing a diffuse growth pattern with three cell types. Neither the SE/SS histotype nor the histopathological features of the tumour correlated with Foxp3+ cell counts. These results indicate that Foxp3+ T reg cells may be associated with a less malignant histological phenotype or may not play a critical role in the immune response of canine seminomas. Moreover, Foxp3+ T reg cells may be associated with SS seminoma, but further studies, involving a larger number of samples, are required to better understand whether these cells play a critical role in the immune response in canine seminomas. This is the first report to demonstrate the characteristics of T reg cell infiltration in canine seminoma.