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Infiltrating Foxp3+ Regulatory T cells and Histopathological Features in Canine Classical and Spermatocytic Seminomas

Authors

  • JH Kim,

    1. Department of Veterinary Pathology, Small Animal Tumour Diagnostic Centre, College of Veterinary Medicine, Konkuk University, Seoul, Korea
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    • Present address: Veterinary Clinical Science, College of Veterinary Medicine, University of Minnesota, St. Paul, MN 55108, USA.

  • SK Chon,

    1. Hyu Animal Clinic, Iksan, Jeollabuk-do, Korea
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  • KS Im,

    1. Department of Veterinary Pathology, Small Animal Tumour Diagnostic Centre, College of Veterinary Medicine, Konkuk University, Seoul, Korea
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  • NH Kim,

    1. Department of Veterinary Pathology, Small Animal Tumour Diagnostic Centre, College of Veterinary Medicine, Konkuk University, Seoul, Korea
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  • KW Cho,

    1. Department of Veterinary Internal Medicine, College of Veterinary Medicine, Gyeongsang National University, Gajwa-dong, Jinju-si, Gyeongsangnam-do, Korea
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  • JH Sur

    1. Department of Veterinary Pathology, Small Animal Tumour Diagnostic Centre, College of Veterinary Medicine, Konkuk University, Seoul, Korea
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Author’s address (for correspondence): JH Sur, Department of Veterinary Pathology, Small Animal Tumour Diagnostic Centre, College of Veterinary Medicine, Konkuk University, 1 Hwayang-dong, Kwangjin-gu, Seoul 143-701, Korea. E-mail: jsur@konkuk.ac.kr

Contents

In humans, regulatory T (T reg) cells are known to play a critical role in both the regulation of immune homoeostasis and the progression of cancer. However, there is little information about the identification, characterization and the function of T reg cells in canine tumours. We identified T reg cells in 28 canine seminoma samples using a Forkhead box P3 (Foxp3) antibody and investigated the relationship between T reg cell infiltration and histopathological features of classical and spermatocytic seminomas (SE and SS, respectively). The Foxp3 protein showed nuclear immunostaining in infiltrating lymphocytes, and Foxp3+ cells were diffused or focally distributed in seminoma tissues. Foxp3+ cells were frequently present in the SS histotype, in seminomas that showed no evidence of tumour cell invasion into the vessels and in seminomas showing a diffuse growth pattern with three cell types. Neither the SE/SS histotype nor the histopathological features of the tumour correlated with Foxp3+ cell counts. These results indicate that Foxp3+ T reg cells may be associated with a less malignant histological phenotype or may not play a critical role in the immune response of canine seminomas. Moreover, Foxp3+ T reg cells may be associated with SS seminoma, but further studies, involving a larger number of samples, are required to better understand whether these cells play a critical role in the immune response in canine seminomas. This is the first report to demonstrate the characteristics of T reg cell infiltration in canine seminoma.

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