Correction added on May 22 2013, after first online publication: The order and affiliations of the authors have been corrected.
Colloidal Centrifugation of Stallion Semen Results in a Reduced Rate of Sperm DNA Fragmentation
Article first published online: 7 JUL 2012
© 2012 Blackwell Verlag GmbH
Reproduction in Domestic Animals
Volume 48, Issue 2, pages e23–e25, April 2013
How to Cite
Crespo, F., Gutiérrez-Cepeda, L., Gosalvez, J., Serres, C. and Johnston, S. (2013), Colloidal Centrifugation of Stallion Semen Results in a Reduced Rate of Sperm DNA Fragmentation. Reproduction in Domestic Animals, 48: e23–e25. doi: 10.1111/j.1439-0531.2012.02140.x
- Issue published online: 12 MAR 2013
- Article first published online: 7 JUL 2012
- Submitted: 17 Apr 2012; Accepted: 26 May 2012
Stallion spermatozoa recovered and examined immediately after colloidal centrifugation resulted in a higher straight-line velocity (VSL) than sperm processed using direct conventional centrifugation (p = 0.000), but there was no differences in the progressive motility or sperm DNA fragmentation (SDF) as determined by the sperm chromatin dispersion assay. However, when centrifuged spermatozoa were incubated at 37°C for 24 h to determine the rate of SDF (r-SDF), a lower r-SDF (p = 0.0011) was observed in those sperm recovered after colloidal separation (0.5 ± 0.1%/h) compared to direct (1.2 ± 0.4%/h) or no centrifugation (r-SDF = 1.2 ± 0.3%/h). These results confirm that colloidal separation of stallion spermatozoa results in prolonged sperm DNA longevity, but these differences were only apparent following a period of incubation and dynamic assessment. Consequently, we strongly recommend the use of the dynamic form of the SDF assay for evaluating centrifugation and/or other ex vivo procedures, as a single basal assessment of SDF may inadvertently result in a false-positive evaluation of DNA quality.