Dinucleotide repeat polymorphisms in the signal transducers and activators of transcription 6 (Stat6) gene in children with allergic diseases
Article first published online: 28 JUN 2008
Volume 53, Issue 3, pages 235–240, September 2004
How to Cite
Suzuki, M., Arakawa, H., Tamura, K., Takizawa, T., Mochizuki, H., Tokuyama, K., Tamari, M., Mao, X.-Q., Shirakawa, T. and Morikawa, A. (2004), Dinucleotide repeat polymorphisms in the signal transducers and activators of transcription 6 (Stat6) gene in children with allergic diseases. Allergology International, 53: 235–240. doi: 10.1111/j.1440-1592.2004.00339.x
- Issue published online: 28 JUN 2008
- Article first published online: 28 JUN 2008
- Received 4 December 2003. Accepted for publication 12 February 2004.
- dinucleotide repeat polymorphism;
- signal transducers and activators of transcription 6 (Stat6)
Background: Signal transducers and activators of transcription 6 (Stat6) is involved in the interleukin (IL)-4 and IL-13 signaling pathway. The aim of the present study was to evaluate whether polymorphisms in the 5′-flanking region of the Stat6 gene are associated with allergic diseases in Japanese children.
Methods: The Stat6 gene polymorphisms were genotyped by polymerase chain reaction (PCR) fragment length polymorphism analysis and the IL-4 receptor Ile50Val polymorphism was examined using PCR methods.
Results: Novel dinucleotide repeat polymorphisms were found in the 5′-flanking sequences, positioning at −865 (Pro A) and −690 (Pro B) of the Stat6 gene starting codon. The GT repeats in the 5′-flanking sequences were highly polymorphic (Pro A: 14−29 repeats; ProB: 14−20 repeats) with no significant differences in the frequency of allelic and genotypic distributions observed between allergic subjects and controls. In the GT repeat polymorphism of Stat6 exon 1, there was a significant difference in the frequency of genotypic distribution between the two groups (P = 0.003). The Stat6 exon 1 variant had significant linkage disequilibrium with Pro B variants, but not with Pro A. The GT repeat polymorphism was not associated with the IL-4 receptor Ile50Val polymorphism.
Conclusions: Variants of the Stat6 gene may be useful markers for predicting allergic diseases in Japanese children.