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Dinucleotide repeat polymorphisms in the signal transducers and activators of transcription 6 (Stat6) gene in children with allergic diseases

Authors


Dr Hirokazu Arakawa, Department of Pediatrics and Developmental Medicine, Gunma University Graduate School, Maebashi, Gunma 371-8511, Japan. Email: harakawa@showa.gunma-u.ac.jp

Abstract

Background:  Signal transducers and activators of transcription 6 (Stat6) is involved in the interleukin (IL)-4 and IL-13 signaling pathway. The aim of the present study was to evaluate whether polymorphisms in the 5′-flanking region of the Stat6 gene are associated with allergic diseases in Japanese children.

Methods:  The Stat6 gene polymorphisms were genotyped by polymerase chain reaction (PCR) fragment length polymorphism analysis and the IL-4 receptor Ile50Val polymorphism was examined using PCR methods.

Results:  Novel dinucleotide repeat polymorphisms were found in the 5′-flanking sequences, positioning at −865 (Pro A) and −690 (Pro B) of the Stat6 gene starting codon. The GT repeats in the 5′-flanking sequences were highly polymorphic (Pro A: 14−29 repeats; ProB: 14−20 repeats) with no significant differences in the frequency of allelic and genotypic distributions observed between allergic subjects and controls. In the GT repeat polymorphism of Stat6 exon 1, there was a significant difference in the frequency of genotypic distribution between the two groups (P = 0.003). The Stat6 exon 1 variant had significant linkage disequilibrium with Pro B variants, but not with Pro A. The GT repeat polymorphism was not associated with the IL-4 receptor Ile50Val polymorphism.

Conclusions:  Variants of the Stat6 gene may be useful markers for predicting allergic diseases in Japanese children.

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