TETRANDRINE AS A CALCIUM ANTAGONIST

Authors


Dr H Takemura, Department of Pharmacology, School of Medicine, Sapporo Medical University, South 1, West 17, Sapporo 060, Japan.

SUMMARY

1. The Ca2+-antagonism of tetrandrine (TET) on the Ca2+ mobilization in various types of cells were reviewed. Inositol trisphosphate (IP3)-generating drugs were used as Ca2+-mobilizing agonists and the effects were compared with those produced by using the microsomal Ca2+-ATPase inhibitor thapsigargin (TG), which is a tool for analysing Ca2+ which is a tool for analysing Ca2+ store -regulated Ca2+ entry (capacitative Ca2+ entry).

2. In rat phaeochromocytoma PC12 cells, 100 μmol/L TET abolished high K+ (30 mmol/L)-induced sustained increases in cytoplasmic Ca2+ concentrations ([Ca2+]i) and partially inhibited bradykinin (1 μmol/L)- or TG (100 nmol/L)-induced Ca2+ entry.

3. In NIH/3T3 fibroblasts and rat parotid acinar cells, 100 μmol/L TET abolished Ca2+ entry induced by bombesin (1 μmol/L) and carbachol (100 μmol/L), respectively, or TG (100 nmol/L). However, in the human leukaemia T cell line Jurkat, 100 μmol/L TET did not inhibit Ca2+ entry evoked by either the anti-CD3 antibody OKT3 (10 mg/L) or TG (100 nmol/L).

4. In rat glioma C6 cells, the effects of TET on Ca2+ mobilization were further examined. At a high concentration, TET (300 μmol/L) alone did not affect [Ca2+]i in C6 cells. Tetrandrine inhibited the peak and sustained increases in [Ca2+]i induced by bombesin and TG in a dose-dependent manner. Although TET or TG did not produce increases in IP3, TET did inhibit increases in IP3 produced by bombesin.

5. Our results suggest that the action of TET on Ca2+ entry is dependent on cell types and that TET inhibits both Ca2+ entry from the extracellular medium and Ca2+ release from intracellular stores in rat glioma C6 cells.

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