Presented at the Australian Physiological and Pharmacological Society (APPS) Symposium Functional Imaging, September 2003. The papers in these proceedings were peer reviewed under the supervision of the APPS Editor. The papers are being published with the permission of the APPS and were initially published on the APPS website (http://www.apps.org.au).
Microscopic imaging of extended tissue volumes
Article first published online: 17 JAN 2005
Clinical and Experimental Pharmacology and Physiology
Volume 31, Issue 12, pages 902–905, December 2004
How to Cite
LeGrice, I., Sands, G., Hooks, D., Gerneke, D. and Smaill, B. (2004), Microscopic imaging of extended tissue volumes. Clinical and Experimental Pharmacology and Physiology, 31: 902–905. doi: 10.1111/j.1440-1681.2004.04101.x
- Issue published online: 17 JAN 2005
- Article first published online: 17 JAN 2005
- Received 27 July 2004; revision 17 August 2004; accepted 7 September 2004.
- cardiac ventricle;
- extended volume;
- laser scanning confocal microscope;
- three-dimensional image
1. Detailed information about three-dimensional structure is key to understanding biological function.
2. Confocal laser microscopy has made it possible to reconstruct three-dimensional organization with exquisite resolution at cellular and subcellular levels.
3. There have been few attempts to acquire large image volumes using the confocal laser scanning microscope.
4. Previously, we have used manual techniques to construct extended volumes (several mm in extent, at 1.5 µm voxel size) of myocardial tissue.
5. We are now developing equipment and efficient automated methods for acquiring extended morphometric databases using confocal laser scanning microscopy.