EFFECT OF LOW-VOLTAGE ELECTRICAL STIMULATION ON ANGIOGENIC GROWTH FACTORS IN ISCHAEMIC RAT SKELETAL MUSCLE
Article first published online: 21 JUN 2006
Clinical and Experimental Pharmacology and Physiology
Volume 33, Issue 7, pages 623–627, July 2006
How to Cite
Nagasaka, M., Kohzuki, M., Fujii, T., Kanno, S., Kawamura, T., Onodera, H., Itoyama, Y., Ichie, M. and Sato, Y. (2006), EFFECT OF LOW-VOLTAGE ELECTRICAL STIMULATION ON ANGIOGENIC GROWTH FACTORS IN ISCHAEMIC RAT SKELETAL MUSCLE. Clinical and Experimental Pharmacology and Physiology, 33: 623–627. doi: 10.1111/j.1440-1681.2006.04417.x
- Issue published online: 21 JUN 2006
- Article first published online: 21 JUN 2006
- Received 29 September 2005; revision 16 December 2005; accepted 26 December 2005.
- angiogenic factors;
- electrical stimulation;
- skeletal muscle
- 1Low-voltage electrical stimulation (LVES) in skeletal muscle at a level far below the threshold of muscle contraction has been reported to promote local angiogenesis. However, the mechanism underlying the promotion of local angiogenesis by LVES has not been fully elucidated. In the present study, we evaluated whether angiogenic factors, such as vascular endotherial growth factor (VEGF), hepatocyte growth factor (HGF) and fibroblast growth factor (FGF), as well as other disadvantageous factors, such as inflammation (interleukin (IL)-6) and hypoxia (hypoxia-inducible factor (HIF)-1α), contribute to the local angiogenesis produced by LVES.
- 2We completely excised bilateral femoral arteries of male Sprague-Dawley rats. After the operation, electrodes were implanted onto the centre of the fascia of the bilateral tibialis anterior (TA) muscles, tunnelled subcutaneously and exteriorized at the level of the scapulae. The right TA muscles of rats were stimulated continuously at a stimulus frequency of 50 Hz, with a 0.1 V stimulus strength and no interval, for 5 days. The left TA muscles served as controls.
- 3We found that both VEGF and HGF protein were significantly increased by LVES in stimulated muscles compared with control. The VEGF level of the LVES group was 89.10 ± 17.19 ng/g, whereas that of the control group was 65.07 ± 12.88 ng/g, as determined by ELISA (P < 0.05). The HGF level of the LVES and control groups was 8.52 ± 1.96 and 5.80 ± 2.14 ng/g, respectively (P < 0.05). In contrast, there was no difference in FGF, IL-6 and HIF-1α between the LVES and control groups.
- 4These results suggest that LVES in a hindlimb ischaemia model of rats increases not only VEGF, but also HGF, production, which may be the main mechanism responsible for the angiogenesis produced by LVES.