Get access

BEHAVIOURAL TESTING AND HISTOLOGICAL ASSESSMENTS OF RABBIT SPINAL CORD FOLLOWING INTRATHECAL ADMINISTRATION OF ONDANSETRON

Authors


Assistant Professor Hector J Lacassie, Departamento de Anestesiología, Pontificia Universidad Católica de Chile, Marcoleta 367, 3rd floor, Santiago 833–0024, Chile. Email: hlacassie@gmail.com

SUMMARY

  • 1Intrathecal injection of ondansetron has the potential to reduce opioid-related side-effects. The aim of the present study was to determine whether this route of administration produces neuraxial injury.
  • 2Adult, non-pregnant female New Zealand white rabbits received a single bolus injection of a low (40 mg) or high (4.0 mg) dose of ondansetron into the intrathecal space between the 4th and 5th lumbar vertebrae. In some cases, ondansetron was coadministered with morphine (5 mg/kg). Control animals received a bolus injection of normal saline. Behavioural assessments were conducted at 1 and 24 h to determine overt changes in arousal and mobility, followed by histological evaluation of the excised spinal cord.
  • 3Of 45 animals investigated, 10 rabbits exhibited modest behavioural evidence of spinal injury, the incidence of which was equally distributed between the treatment groups. Haematoxylin and eosin, along with HAM56, staining of cross-sections of the cervical, thoracic and upper and lower lumbar areas revealed mild signs of inflammation. This, too, was equally distributed between the treatment groups, suggesting that any observed neuraxial injury was the result of needle trauma and not ondansetron neurotoxicity.
  • 4Collectively, these negative findings support conducting further experiments to fully assess the clinical usefulness of intrathecal ondansetron administration.
Get access to the full text of this article

Ancillary