IMIQUIMOD, A TOLL-LIKE RECEPTOR 7 LIGAND, INHIBITS AIRWAY REMODELLING IN A MURINE MODEL OF CHRONIC ASTHMA
Article first published online: 28 AUG 2008
© 2008 The Authors. Journal compilation © 2008 Blackwell Publishing Asia Pty Ltd
Clinical and Experimental Pharmacology and Physiology
Volume 36, Issue 1, pages 43–48, January 2009
How to Cite
Du, Q., Zhou, L.-F., Chen, Z., Gu, X.-Y., Huang, M. and Yin, K.-S. (2009), IMIQUIMOD, A TOLL-LIKE RECEPTOR 7 LIGAND, INHIBITS AIRWAY REMODELLING IN A MURINE MODEL OF CHRONIC ASTHMA. Clinical and Experimental Pharmacology and Physiology, 36: 43–48. doi: 10.1111/j.1440-1681.2008.05027.x
- Issue published online: 18 JAN 2009
- Article first published online: 28 AUG 2008
- Received 9 November 2007; revision 6 June 2008; accepted 25 June 2008.
- airway remodelling;
- Toll-like receptor
- 1Imiquimod, a synthetic Toll-like receptor (TLR) 7 ligand, has been shown to attenuate airway inflammation and airway hyperresponsiveness (AHR) in acute murine models of allergic asthma. In the present study, we investigated the effect of imiquimod on allergen-induced airway remodelling in chronic experimental asthma.
- 2Ovalbumin (OVA)-sensitized mice were chronically challenged with aerosolized OVA for 8 weeks. Some mice were exposed to an aerosol of 0.15% imiquimod daily during the period of OVA challenge. Twenty-four hours after the last OVA challenge, mice were evaluated for the development of airway inflammation, AHR and airway remodelling. The levels of total serum IgE and Th2 cytokines (interleukin (IL)-4, IL-5 and IL-13) in bronchoalveolar lavage fluid (BALF) and the expression of transforming growth factor (TGF)-β1 protein in lungs were measured by ELISA and immunohistochemistry, respectively.
- 3The results demonstrated that imiquimod significantly inhibited chronic inflammation, persistent AHR and airway remodelling in chronic experimental asthma. In addition, imiquimod reduced levels of total serum IgE and BALF Th2 cytokines and diminished expression of TGF-β1 in remodelled airways.
- 4In summary, the results of the present study indicate that imiquimod may attenuate the progression of airway inflammation and remodelling, providing potential in the treatment of asthma.