These two authors contributed equally to this work.
Administration of midazolam in infancy does not affect learning and memory of adult mice
Article first published online: 19 MAY 2009
© 2009 The Authors. Journal compilation © 2009 Blackwell Publishing Asia Pty Ltd
Clinical and Experimental Pharmacology and Physiology
Volume 36, Issue 12, pages 1144–1148, December 2009
How to Cite
Xu, H., Liu, Z.-Q., Liu, Y., Zhang, W.-S., Xu, B., Xiong, Y.-C. and Deng, X.-M. (2009), Administration of midazolam in infancy does not affect learning and memory of adult mice. Clinical and Experimental Pharmacology and Physiology, 36: 1144–1148. doi: 10.1111/j.1440-1681.2009.05208.x
- Issue published online: 25 NOV 2009
- Article first published online: 19 MAY 2009
- Received 21 August 2008; revision 9 April 2009; accepted 14 April 2009.
- learning and memory;
- novel object recognition;
- passive avoidance test;
- 1Midazolam is a common fast-acting GABAA receptor agonist. Recent data suggest that exposure to midazolam in early life may cause long-term effects on brain function through stable epigenetic reprogramming. The aim of the present study was to determine whether the administration of midazolam to infant mice would affect their learning and memory in adulthood.
- 2An open-field test was conducted before and then 3, 24, 48 and 72 h after administration of midazolam (50 mg/kg, i.p.) to infant mice. Saline control mice received an equal volume of saline i.p. 3 h before the open-field test. Total movements, total movement time, total movement distance and velocity were analysed. Novel object recognition (NOR), Morris water-maze and passive avoidance tests were performed when the treated mice grew to adulthood (105 days of age).
- 3The results of open-field test showed that midazolam significantly reduced locomotor activity (total movements, total movement time, total movement distance and velocity) in infant mice 3 and 24 h after drug administration and that these effects had disappeared by 72 h after drug administration. The results of the water-maze, NOR and passive avoidance tests in adulthood (at 105 days of age) indicated that administration of midazolam in infancy had no long-term effects on the learning and memory behaviours of adult mice compared with the saline control.
- 4Acute midazolam administration to infant mice affected spontaneous locomotor activity for approximately 2 days, but did not seem to have any significant impact on cognitive functioning that lasted into adulthood.