Inhibition of extracellular signal-regulated kinase activity improves cognitive function in Tg2576 mice
Article first published online: 26 SEP 2012
© 2012 The Authors Clinical and Experimental Pharmacology and Physiology © 2012 Wiley Publishing Asia Pty Ltd
Clinical and Experimental Pharmacology and Physiology
Volume 39, Issue 10, pages 852–857, October 2012
How to Cite
Jin, P., Choi, D.-Y. and Hong, J. T. (2012), Inhibition of extracellular signal-regulated kinase activity improves cognitive function in Tg2576 mice. Clinical and Experimental Pharmacology and Physiology, 39: 852–857. doi: 10.1111/j.1440-1681.2012.12000.x
- Issue published online: 26 SEP 2012
- Article first published online: 26 SEP 2012
- Manuscript Accepted: 1 AUG 2012
- Manuscript Revised: 30 JUL 2012
- Manuscript Received: 5 APR 2012
- Korean Government
- National Research Foundation of Korea (NRF). Grant Numbers: 0029480, A101836, 2010–0029480
- Ministry of Education, Science and Technology. Grant Number: 2011–0031403
- Alzheimer's disease;
- extracellular signal-regulated kinase inhibitor;
- memory function;
- Deposition of β-amyloid (Aβ) peptide is a defining pathological hallmark of Alzheimer's disease (AD) and is involved in memory impairment. Evidence suggests that activation of an extracellular signal-regulated kinase (ERK) pathway is related to Aβ accumulation. Thus, the aim of the present study was to investigate the effects of an ERK inhibitor (U0126) on amyloidogenesis and cognitive function in Tg2576 mice.
- Tg2576 mice were injected with U0126 (20 mg/kg, i.p.) or vehicle (1% dimethyl sulphoxide in sterile saline) once a day for 7 days and then cognitive function was assessed by the Morris water maze test and passive avoidance test. In addition, immunostaining, western blot analysis, ELISA and enzyme activity assays were used to examine the degree of Aβ deposition in the brains of Tg2576 mice.
- Our results showed that U0126 attenuated memory impairment and inhibited Aβ deposition in the brains of Tg2576 mice. Further experiments revealed that the inhibition of Aβ deposition by U0126 was due to a reduction in β-secretase and amyloid precursor protein expression in the brains of U0126-treated Tg2576 mice.
- These results suggest that the ERK pathway is associated with Aβ accumulation and consequent memory dysfunction in Tg2576 mice and that inhibition of the ERK pathway may be an appropriate intervention in the treatment of AD.