Morphological Modifications of Oocyte Vitelline Envelope from Bufo arenarum during Different Functional States

(vitelline envelope/amphibian eggs/fertilization)

Authors

  • MARTA I. MARIANO,

    Corresponding author
    1. Laboratorio de Investigaciones Embriológicas (LABINE). Consejo National de Investigaciones Cientificas y Técnicas. Buenos Aires, República Argentina
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  • MARTA G. De MARTIN,

    1. Institute de Biologia. Facultad de Bioquímica, Quimica y Farmacia. Universidad de Tucumán, República Argentina
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  • ARMANDO PISANO

    1. Laboratorio de Investigaciones Embriológicas (LABINE). Consejo National de Investigaciones Cientificas y Técnicas. Buenos Aires, República Argentina
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For reprint request, reffer to: Marta I. Mariano-LABINE (CONICET)-Montevideo 126, 1019 Buenos Aires-Rep. Argentina

Abstract

The submicroscopic morphology of the vitelline envelope of Bufo arenarum's oocyte change significantly during the maturation and fertilization processes. The morphological changes are related to physiological activity in vivo and can be triggered in vitro by experimental procedures. It is our scope to present the ultrastructure differences of fascicular components of the vitelline envelope in coelomic, “pars recta” conditioned, oviductal, oviposited and fertilized oocytes. Our experimental results indicate that artificial “pars recta” treatment of coelomic oocytes arrange the fascicular components as those of oviposited oocyte, although differences still remain indicating that additional maturation processes take place while the egg pass througth the oviduct. Fertilized or activated oocytes which are refractary to sperm penetration, change the vitelline envelope fascicular components orientation giving a submicroscopical image quite different to those of none fertilized oocytes. These ultrastructural changes define in a clear cut manner the functional states of Bufo arenarum's oocyte.

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