Direct Evidence for the Presence of Germ Cell Determinant in Vegetal Pole Cytoplasm of Xenopus laevis and in a Subcellular Fraction of It
(Xenopus laevis/germ cell determinant/germ plasm/PGC induction)
Article first published online: 7 JUL 2008
Development, Growth & Differentiation
Volume 28, Issue 6, pages 563–568, November 1986
How to Cite
IKENISHI, K., NAKAZATO, S. and OKUDA, T. (1986), Direct Evidence for the Presence of Germ Cell Determinant in Vegetal Pole Cytoplasm of Xenopus laevis and in a Subcellular Fraction of It. Development, Growth & Differentiation, 28: 563–568. doi: 10.1111/j.1440-169X.1986.00563.x
- Issue published online: 7 JUL 2008
- Article first published online: 7 JUL 2008
- (Received March 15, 1986; accepted July 3. 1986)
To test for the presence of germ cell determinant in Xenopus embryos, vegetal pole cytoplasm containing the “germ plasm”, or a subcellular fraction of it, was microinjected into single somatic blastomeres isolated from 32-cell embryos. Injected or non-injected (control) blastomeres were cultured in 3H-thymidine until normal control embryos reached the neurula stage. The labeled explants were then implanted into unlabeled host neurulae, which were allowed to develop to the tadpole stage. Labeled PGCs of explant origin in the genital ridges of the experimental tadpoles were examined by autoradiography.
Isolated blastomeres were injected with vegetal pole cytoplasm of 32-cell embryos or with a 20,000 g pellet made from vegetal pole cytoplasm of 2-cell embryos. Labeled PGCs were found in 7.6% and 2.3% of the experimental tadpoles, respectively. No labeled PGCs were found in the control tadpoles, except for one tadpole in the first experiment. These results strongly suggest that the vegetal pole cytoplasm and its subcellular fractions act as germ cell determinant.