• 1
    Chesley, P., 1935. Development of the short-tailed mutation in the house mouse. J. Exp. Zool., 70, 429459.
  • 2
    Chomczynski, P. and N. Sacchi, 1987. Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem., 162, 156159.
  • 3
    Cloney, R. A., 1964. Development of the ascidian notochord. Acta Embryol. Morphol. Exp., 7, 111130.
  • 4
    Conklin, E. G., 1905. The organization and cell lineages of the ascidian egg. J. Acad. Natl. Sci. (Philad.), 13, 1119.
  • 5
    Crowther, R. J. and J. R. Whittaker, 1986. Developmental autonomy of presumptive notochord cells in partial embryos of an ascidian. Int. J. Invertebrate Reprod. Dev., 9, 253261.
  • 6
    Cunliffe, V. and J. C. Smith, 1992. Ectopic mesoderm formation in Xenopus embryos caused by widespread expression of a Brachyury homologue. Nature, 358, 427430.
  • 7
    Green, J. B. A., H. V. New and J. C. Smith, 1992. Responses of embryonic Xenopus cells to activin and FGF are separated by multiple dose thresholds and correspond to distinct axes of the mesoderm. Cell, 71, 731739.
  • 8
    Gruneberg, H., 1958. Genetical studies on the skeleton of the mouse XXIII: The development of Brachyury and Anury. J. Embryol. Exp. Morphol., 6, 424443.
  • 9
    Herrmann, B. G., S. Labeit, A. Poustka, T. R. King and H. Lehrach, 1990. Cloning of the T gene required in mesoderm formation in the mouse. Nature, 343, 617622.
  • 10
    Holland, P. W. H., L. Z. Holland, N. A. Williams and N. D. Holland, 1992. An amphioxius homeobox gene: sequence conservation, spatial expression during development and insights into vertebrate evolution. Development, 116, 653661.
  • 11
    Jefferies, R. P. S., 1986. The Ancestry of the Vertebrates. London : British Museum.
  • 12
    Katz, M. J., 1983. Comparative anatomy of the tunicate tadpole, Ciona intestinalis. Biol. Bull. 164, 127.
  • 13
    Kowalevsky, A., 1866. Entwicklungsgeschichte der einfachen Ascidien. Mem. l'Acad. St. Petersbourg, Ser. 7, 10, 119.
  • 14
    Kusakabe, T., K. W. Makabe and N. Satoh, 1992. Tunicate muscle actin genes. Structure and organization as a gene cluster. J. Mol. Biol., 227, 955960.
  • 15
    Mancuso, V. and G. Dolcemascolo, 1977. Aspetti ultrastrutturali della corda delle larve di Ciona intestinalis durante l'allun-gamento della corda. Acta Embryol. Exp., 2, 207220.
  • 16
    Miyamoto, D. M. and R. J. Crowther, 1985. Formation of the notochord in living ascidian embryos. J. Embryol. Exp. Morphol., 86, 117.
  • 17
    Nishida, H. and N. Satoh, 1985. Cell lineage analysis in ascidian embryos by intracellular injection of a tracer enzyme. II. The 16- and 32-cell stages. Develop. Biol., 110, 440454.
  • 18
    Nishida, H., 1987. Cell lineage analysis in ascidian embryos by intracellular injection of a tracer enzyme. III. Up to the tissue-restricted stage. Develop. Biol., 121, 526541.
  • 19
    Nishida, H., 1992. Developmental potential for tissue differentiation of fully dissociated cells of the ascidian embryo. Roux's Arch. Dev. Biol., 201, 8187.
  • 20
    Nishikata, T. and N. Satoh, 1990. Specification of notochord cells in the ascidian embryo analysed with a specific monoclonal antibody. Cell Differ. Dev., 30, 4353.
  • 21
    Ortolani, G., 1957. II territorio precoce della corda nelle ascidie. Acta Embryol. Morphol. Exp., 1, 3336.
  • 22
    Pflugfelder, G. O., H. Roth and B. Poeck, 1992. A homology domain shared between Drosophila optomotor-blind and mouse Brachyury is involved in DNA binding. Biochem. Biophys. Res. Commun., 186, 918925.
  • 23
    Rashbass, P., L. A. Cooke, B. G. Herrmann and R. S. P. Beddington, 1991. A cell autonomous function of Brachyury in T/T embryonic stem cell chimaeras. Nature, 353, 348351.
  • 24
    Reverberi, G. and A. Minganti, 1946. Fenomeni di evocazione nello sviluppo di Ascidiella aspersa e di Ascidia malaca allo stadio di otto blastomeri. Publ. Staz. Zool. Napoli, 20, 199252.
  • 25
    Sambrook, J., E. F. Fritsch and T. Maniatis, 1989. Molecular Cloning: A Laboratory Manual, 2nd ed. Cold Spring Harbor Laboratory, New York :.
  • 26
    Sanger, F., S. Nicklen and A. R. Coulson, 1977. DNA sequencing with chain-terminating inhibitors. Proc. Natl. Acad. Sci. U.S.A., 74, 54635467.
  • 27
    Satoh, N., 1993. Developmental Biology of Ascidians. Cambridge University Press, New York , in press.
  • 28
    Schulte-Merker, S., R. K. Ho, B. G. Herrmann and C. Nusslein-Volhard, 1992. The protein product of the zebrafish homologue of the mouse T gene is expressed in nuclei of the germ ring and the notochord of the early embryo. Development, 116, 10211032.
  • 29
    Slack, J. M. W., 1991. From Egg to Embryo, 2nd ed. Cambridge Univ. Press, Cambridge .
  • 30
    Smith, J. C., B. M. J. Price, J. B. A. Green, D. Weigel and B. G. Herrmann, 1991. Expression of a Xenopus homolog of Brachyury (T) is an immediate-early response to mesoderm induction. Cell, 67, 7987.
  • 31
    Spemann, H. and H. Mangold, 1924. Uber Induktion von Embryonalanlagen durch Implantation artfremder Organisatoren. Arch. Mikrosk. Anat. EntwMech., 100, 599638.
  • 32
    Stott, D., A. Kispert and B. G. Herrmann, 1993. Rescue of the tail defect of Brachyury mice. Genes Dev. 7, 197203.
  • 33
    Wilkinson, D. G., S. Bhatt and B. G. Herrmann, 1990. Expression pattern of the mouse T gene and its role in mesoderm formation. Nature, 343, 657659.
  • 34
    Willmer, P., 1990. Invertebrate Relationships. Cambridge University Press, Cambridge .
  • 35
    Yanagisawa, K. O., H. Fujimoto and H. Urushihara, 1981. Effects of the Brachyury (T) mutation on morphogenetic movement in the mouse embryo. Develop. Biol., 87, 242248.
  • 36
    Yasuo, H. and N. Satoh, 1993. Function of vertebrate T gene. Nature, 364, 582583.