Further extension of mammalian GATA-6
Article first published online: 30 NOV 2005
Development, Growth & Differentiation
Volume 47, Issue 9, pages 591–600, December 2005
How to Cite
Maeda, M., Ohashi, K. and Ohashi-Kobayashi, A. (2005), Further extension of mammalian GATA-6. Development, Growth & Differentiation, 47: 591–600. doi: 10.1111/j.1440-169X.2005.00837.x
- Issue published online: 30 NOV 2005
- Article first published online: 30 NOV 2005
- Received 8 October 2005; accepted 14 October 2005.
- DNA binding;
- protein degradation;
- zinc finger
Mammalian GATA-6, which has conserved tandem zinc fingers (CVNC-X17-CNAC)-X29-(CXNC-X17-CNAC), is essential for the development and specific gene regulation of the heart, gastrointestinal tract and other tissues. GATA-6 recognizes the (A/T/C)GAT(A/T)(A) sequence, and interacts with other transcriptional regulators through its zinc-finger region. The mRNA of GATA-6 uses two Met codons in frame as translational initiation codons, and produces L- and S-type GATA-6 through leaky ribosome scanning. GATA-6 is subjected to cAMP-dependent proteolysis by a proteasome in a heterologous expression system. These protein-based characteristics of GATA-6 will be helpful for the identification of target genes, together with determination of the in vivo binding sites for GATA-6 and understanding of the complex network of gene regulation mediated by GATA-6.