Drosophila NAT1, a homolog of the vertebrate translational regulator NAT1/DAP5/p97, is required for embryonic germband extension and metamorphosis
Article first published online: 17 AUG 2007
Development, Growth & Differentiation
Volume 49, Issue 7, pages 623–634, September 2007
How to Cite
Yoshikane, N., Nakamura, N., Ueda, R., Ueno, N., Yamanaka, S. and Nakamura, M. (2007), Drosophila NAT1, a homolog of the vertebrate translational regulator NAT1/DAP5/p97, is required for embryonic germband extension and metamorphosis. Development, Growth & Differentiation, 49: 623–634. doi: 10.1111/j.1440-169X.2007.00956.x
- Issue published online: 17 AUG 2007
- Article first published online: 17 AUG 2007
- Received 21 January 2007; revised 18 June 2007; accepted 20 June 2007.
- Drosophila development;
- germband extension;
- translational regulator.
Translational regulation has been to shown to play major roles in the patterning of the early Drosophila embryo. The eIF4G family member NAT1/p97/DAP5 has been identified as a novel translational repressor. To genetically dissect the in vivo function of this unconventional eIF4G-related translational regulator, Drosophila NAT1 (dNAT1) mutants were isolated using a reverse-genetics approach. Four transposon insertion mutants and a deletion mutant affecting the dNAT1 locus were analyzed. Genetic complementation tests and germline rescue using a 12 kb dNAT1 genomic DNA fragment revealed these to be loss-of-function mutants. One P-element insertion line, dNAT1GS1., shows severe embryonic lethality and abnormal germband extension. Abnormalities at metamorphosis were also found, including defective head eversion and salivary gland degeneration in the hypomorphic allele dNATex1. A phenotypic analysis of dNAT1 mutants suggests that dNAT protein plays a specific rather than general role in translational regulation.