Retrovirus vector-mediated gene transfer into the chick optic vesicle by in ovo electroporation
Article first published online: 28 APR 2008
© 2008 The Authors. Journal compilation © 2008 Japanese Society of Developmental Biologists
Development, Growth & Differentiation
Special Issue: Innovative techniques for the study of development
Volume 50, Issue 6, pages 453–457, August 2008
How to Cite
Sakuta, H., Suzuki, R. and Noda, M. (2008), Retrovirus vector-mediated gene transfer into the chick optic vesicle by in ovo electroporation. Development, Growth & Differentiation, 50: 453–457. doi: 10.1111/j.1440-169X.2008.01021.x
- Issue published online: 4 JUL 2008
- Article first published online: 28 APR 2008
- Received 25 January 2008; revised 29 January 2008; accepted 27 February 2008.
- in ovo electroporation;
- retrovirus vector
Owing to its external position in the embryo, the chick eye has been used as a readily accessible model for studying the molecular mechanisms behind the patterning of the central nervous system. Although methods of genetic analysis have not been established as in the mouse, the chick is convenient for analyzing the functions of genes by in ovo electroporation of retroviral vectors. In this review, we describe the retroviral vector-mediated transfer of genes into the chick optic vesicle by in ovo electroporation. A rapid, efficient, and sustained expression of transgenes is achieved by this approach.