Role of metalloproteinases at the onset of liver development
Article first published online: 28 APR 2008
© 2008 The Authors. Journal compilation © 2008 Japanese Society of Developmental Biologists
Development, Growth & Differentiation
Volume 50, Issue 5, pages 331–338, June 2008
How to Cite
Margagliotti, S., Clotman, F., Pierreux, C. E., Lemoine, P., Rousseau, G. G., Henriet, P. and Lemaigre, F. P. (2008), Role of metalloproteinases at the onset of liver development. Development, Growth & Differentiation, 50: 331–338. doi: 10.1111/j.1440-169X.2008.01031.x
- Issue published online: 28 APR 2008
- Article first published online: 28 APR 2008
- Received 5 December 2007; revised 11 February 2008; accepted 27 February 2008.
- hepatoblast migration;
- liver development;
- matrix metalloproteinases
At the onset of liver development, the hepatic precursor cells, namely, the hepatoblasts, derive from the ventral foregut endoderm and form a bud surrounded by a basement membrane (BM). To initiate liver growth, the hepatoblasts migrate across the BM and invade the neighboring septum transversum mesenchyme. In the present study, carried out in the mouse embryo, we searched for effectors involved in this process and we examined the role of matrix metalloproteinases (MMPs). We found expression of a broad range of MMPs, among which MMP-2 was predominantly expressed in the septum transversum and MMP-14 in the hepatoblasts. Using a new liver explant culture system we showed that inhibition of MMP activity represses migration of the hepatoblasts. We conclude that MMPs are required to initiate expansion of the liver during development and that our culture system provides a new model to study hepatoblast migration.