Application of heat shock promoter in transgenic zebrafish
Article first published online: 22 APR 2008
© 2008 The Authors. Journal compilation © 2008 Japanese Society of Developmental Biologists
Development, Growth & Differentiation
Special Issue: Innovative techniques for the study of development
Volume 50, Issue 6, pages 401–406, August 2008
How to Cite
Shoji, W. and Sato-Maeda, M. (2008), Application of heat shock promoter in transgenic zebrafish. Development, Growth & Differentiation, 50: 401–406. doi: 10.1111/j.1440-169X.2008.01038.x
- Issue published online: 4 JUL 2008
- Article first published online: 22 APR 2008
- Received 19 February 2008; accepted 28 February 2008.
The heat shock promoter is useful for regulating transgene expression in small water-living organisms. In zebrafish embryos, downstream gene expression can be greatly induced throughout the body by raising the temperature from 28.5°C to 38.0°C. By manipulating the local temperature within an embryo, spatial control of transgene expression is also possible. One such way for inducing heat shock response in targeted cells is by using a laser microbeam under the microscope. In addition, random mosaic expression by transient gene expression and transplantation of the transgenic embryo into a wild type host can be considered a powerful tool for studying gene functions using this promoter. In this paper, we review the applications of the zebrafish heat shock protein promoter as a gene expression tool and for lineage labeling and transcription enhancer screening.